| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-04 |
| タイトル |
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タイトル |
Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: feasibility and outcomes from a multicentre study |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
古谷野, 伸
Inoue, Naoki
Oka, Akira
Moriuchi, Hiroyuki
Asano, Kimisato
Ito, Yushi
Yamada, Hideto
Yoshikawa, Tetsushi
Suzutani, Tatsuo
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| 書誌情報 |
BMJ Open
巻 1,
号 1,
p. e000118,
発行日 2011-07-01
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| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2044-6055 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1136/bmjopen-2011-000118 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background As congenital cytomegalovirus (CMV) infection causes significant clinical consequences not only at birth but also later as neurological sequelae, it is critical to establish a strategy for screening congenitally infected newborns. Previous studies have identified an insufficient sensitivity in screening methods based on the use of dried blood spots (DBSs). Objectives To evaluate the feasibility of the authors' recently developed method for large-scale screening for congenital CMV infection and to identify risk factors for congenital infection. Methods More than 21 000 newborns were enrolled at 25 sites in six geographically separate areas of Japan. Urine was collected onto filter cards placed in the diapers, which were then analysed by quantitative PCR using the filter disc directly as a template. Clinical and physical findings of the newborns were extracted from their medical records. CMV strains from the cases and their siblings were genetically compared. Viral loads in DBSs obtained from some of the cases were compared with those in the urine filters. Results Congenital CMV infection was identified in 0.31% (95% CI 0.24% to 0.39%) of the newborns, and 30% of the cases (20/66) had typical clinical manifestations and/or showed abnormalities in brain images at birth. Although the positive predictive value of our screening was 94%, the lack of any comparison with a gold standard assay prevented calculation of the negative predictive value. Almost two-thirds of the cases had siblings, a significantly higher frequency than for uninfected newborns. Most of the cases (21/25) excreted CMV strains identical to those of their siblings. CMV DNA was undetectable in three out of 12 retrievable DBS specimens. Conclusions Implementation of an effective large-scale screening programme for congenital CMV infection is feasible. Siblings are the major risk factor for congenital CMV infection, which emphasises the need for education of mothers-to-be as well as vaccine development. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Publisher |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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22021766 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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5585 |
5013.pdf (373.1 kB)
