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Shortening of alkaline DNA unwinding time does not interfere with detecting DNA damage to mouse and human spermatozoa in the comet assay
https://asahikawa-med.repo.nii.ac.jp/records/4040
https://asahikawa-med.repo.nii.ac.jp/records/4040de13a51e-f7d0-47bb-b3cc-d85efed42571
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
4494.pdf (187.1 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||
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| 公開日 | 2012-05-17 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Shortening of alkaline DNA unwinding time does not interfere with detecting DNA damage to mouse and human spermatozoa in the comet assay | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
日下部, 博一
× 日下部, 博一
× Tateno, Hiroyuki
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| 書誌情報 |
Asian Journal of Andrology 巻 13, 号 1, p. 172-174, 発行日 2011-01-01 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 1008-682X | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1038/aja.2010.105 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The comet assay was performed on mouse and human spermatozoa to examine the effect of alkaline DNA unwinding time. The spermatozoa were treated in vitro with the DNA-damaging agents, methyl methanesulfonate (MMS) or hydrogen peroxide (H2O2), and then embedded in agarose gel on glass slides. The slides were immersed in alkaline solution (>pH 13) for 1, 5, 10 and 20 min, and then subjected to the electrophoresis under neutral conditions. In mouse spermatozoa, comet tails seen in solvent controls became brighter and longer as the alkaline DNA unwinding time increased. However, in the MMS-treated mouse spermatozoa, a smaller difference in the damage from that in the solvent control was seen with time within a dose. DNA damage induced by H2O2 could also be detected accurately after alkali treatment for 1–20 min. In human spermatozoa, DNA damage induced by MMS and H2O2 could be detected in a dose-dependent manner after alkali treatment for 1 min. The ability of the comet assay to detect DNA damage was not adversely affected by the short period (1 min) of the alkaline DNA unwinding time. | |||||||||||
| 言語 | en | |||||||||||
| 注記 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 注記 | http://dx.doi.org/10.1038/aja.2010.105 | |||||||||||
| 資源タイプ | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 資源タイプ | text | |||||||||||
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| 出版タイプ | AM | |||||||||||
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| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| ID(XooNIps) | ||||||||||||
| 21057514 | ||||||||||||
| 閲覧数(XooNIps) | ||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||
| 995 | ||||||||||||
