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Characterization of chromosomal damage accumulated in freeze-dried mouse spermatozoa preserved under ambient and heat stress conditions

https://asahikawa-med.repo.nii.ac.jp/records/3979
https://asahikawa-med.repo.nii.ac.jp/records/3979
55a30453-3b47-4cb3-8b96-4326550d3078
名前 / ファイル ライセンス アクション
4729.pdf 4729.pdf (274.0 kB)
Item type 学術雑誌論文 / Journal Article_02(1)
公開日 2012-05-30
タイトル
タイトル Characterization of chromosomal damage accumulated in freeze-dried mouse spermatozoa preserved under ambient and heat stress conditions
言語 en
言語
言語 eng
資源タイプ
資源タイプ journal article
著者 日下部, 博一

× 日下部, 博一

ja 日下部, 博一

ja-Kana クサカベ, ヒロカズ

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立野, 裕幸

× 立野, 裕幸

ja 立野, 裕幸

ja-Kana タテノ, ヒロユキ

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著者 ローマ字
Tateno, Hiroyuki
著者 ローマ字
en
書誌情報 Mutagenesis

巻 26, 号 3, p. 447-453, 発行日 2011-05-01
ISSN
収録物識別子タイプ PISSN
収録物識別子 0267-8357
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 10.1093/mutage/ger003
リンクURL
内容記述タイプ Other
内容記述 http://www.ncbi.nlm.nih.gov/pubmed/21367815 | http://www.ncbi.nlm.nih.gov/pubmed/21367815
抄録
内容記述タイプ Abstract
内容記述 Structural chromosome aberrations and DNA damage generated in freeze-dried mouse spermatozoa were investigated. Freeze-dried sperm samples were preserved at 4, 25 and 50℃ for short duration (1 day to 2 months) and at 25℃ for long duration (2 years). The spermatozoa were injected into mouse oocytes to analyse the chromosomes of the zygotes at the first cleavage metaphase. Chromosome break of the chromosome-type aberrations was the most common type of structural chromosome aberrations observed in all freeze-dried samples. The frequency of chromatid exchanges rapidly increased in freeze-dried spermatozoa preserved at 50℃ for 1-5 days. The frequency of chromatid-type aberrations (break and exchange) gradually increased in freeze-dried spermatozoa preserved at 25℃ for up to 2 months. Alkaline comet assay revealed significant migration of damaged DNA accumulated in freeze-dried spermatozoa preserved at 50℃ for 3 days and 25℃ for 2 years. However, no DNA damage was detected using the same sperm samples by neutral comet assay, which can detect mostly DNA double-strand breaks in cellular DNA. These results suggest that DNA single-strand breaks were accumulated in freeze-dried spermatozoa preserved under ambient or heat conditions, and then chromatid-type aberrations, especially the chromatid exchanges, were formed via post-replication repair system in zygotes.
言語 en
注記
内容記述タイプ Other
注記 This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version Kusakabe, Hirokazu ; Tateno, Hiroyuki, Mutagenesis, 26(3), 447-453. is available online at: http://mutage.oxfordjournals.org/content/26/3/447.
資源タイプ
内容記述タイプ Other
資源タイプ text
著者版フラグ
出版タイプ AM
フォーマット
内容記述タイプ Other
内容記述 application/pdf
ID(XooNIps)
21367815
閲覧数(XooNIps)
ダウンロード数(XooNIps)
754
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