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Characterization of chromosomal damage accumulated in freeze-dried mouse spermatozoa preserved under ambient and heat stress conditions
https://asahikawa-med.repo.nii.ac.jp/records/3979
https://asahikawa-med.repo.nii.ac.jp/records/397955a30453-3b47-4cb3-8b96-4326550d3078
| 名前 / ファイル | ライセンス | アクション |
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4729.pdf (274.0 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||
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| 公開日 | 2012-05-30 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Characterization of chromosomal damage accumulated in freeze-dried mouse spermatozoa preserved under ambient and heat stress conditions | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
日下部, 博一
× 日下部, 博一
× 立野, 裕幸
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| 著者 ローマ字 | ||||||||||||||
| Tateno, Hiroyuki | ||||||||||||||
| 著者 ローマ字 | ||||||||||||||
| en | ||||||||||||||
| 書誌情報 |
Mutagenesis 巻 26, 号 3, p. 447-453, 発行日 2011-05-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 0267-8357 | |||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1093/mutage/ger003 | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | http://www.ncbi.nlm.nih.gov/pubmed/21367815 | http://www.ncbi.nlm.nih.gov/pubmed/21367815 | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Structural chromosome aberrations and DNA damage generated in freeze-dried mouse spermatozoa were investigated. Freeze-dried sperm samples were preserved at 4, 25 and 50℃ for short duration (1 day to 2 months) and at 25℃ for long duration (2 years). The spermatozoa were injected into mouse oocytes to analyse the chromosomes of the zygotes at the first cleavage metaphase. Chromosome break of the chromosome-type aberrations was the most common type of structural chromosome aberrations observed in all freeze-dried samples. The frequency of chromatid exchanges rapidly increased in freeze-dried spermatozoa preserved at 50℃ for 1-5 days. The frequency of chromatid-type aberrations (break and exchange) gradually increased in freeze-dried spermatozoa preserved at 25℃ for up to 2 months. Alkaline comet assay revealed significant migration of damaged DNA accumulated in freeze-dried spermatozoa preserved at 50℃ for 3 days and 25℃ for 2 years. However, no DNA damage was detected using the same sperm samples by neutral comet assay, which can detect mostly DNA double-strand breaks in cellular DNA. These results suggest that DNA single-strand breaks were accumulated in freeze-dried spermatozoa preserved under ambient or heat conditions, and then chromatid-type aberrations, especially the chromatid exchanges, were formed via post-replication repair system in zygotes. | |||||||||||||
| 言語 | en | |||||||||||||
| 注記 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 注記 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version Kusakabe, Hirokazu ; Tateno, Hiroyuki, Mutagenesis, 26(3), 447-453. is available online at: http://mutage.oxfordjournals.org/content/26/3/447. | |||||||||||||
| 資源タイプ | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 資源タイプ | text | |||||||||||||
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| 出版タイプ | AM | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
| ID(XooNIps) | ||||||||||||||
| 21367815 | ||||||||||||||
| 閲覧数(XooNIps) | ||||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||||
| 754 | ||||||||||||||
