WEKO3
アイテム
A rapid method to isolate soluble royal jelly proteins
https://asahikawa-med.repo.nii.ac.jp/records/3932
https://asahikawa-med.repo.nii.ac.jp/records/39322a25f8a4-c3cd-4f63-9e95-3df05938a6e3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
4653.pdf (1.0 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-07-06 | |||||
| タイトル | ||||||
| タイトル | A rapid method to isolate soluble royal jelly proteins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Royal jelly | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Ultracentrifugation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Soluble royal jelly proteins | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | MRJP1 oligomer | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | HPLC | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
野崎, 怜雄
× 野崎, 怜雄× Tamura, Shogo× Ito, Aimi× Moriyama, Takanori× Yamaguchi, Kikuji× Kono, Toru |
|||||
| 書誌情報 |
Food Chemistry 巻 134, 号 4, p. 2332-2337, 発行日 2012-10-01 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0308-8146 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.foodchem.2012.03.106 | |||||
| リンクURL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science/article/pii/S0308814612005912 | http://www.sciencedirect.com/science/article/pii/S0308814612005912 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Soluble royal jelly (RJ) proteins (SRJPs) include the major RJ protein (MRJP) family, which contribute to the physiological actions of RJ. Although SRJPs are prepared using conventional methods involving dialysis and centrifugation, dialysis is a time-consuming process. We have therefore developed a simple method to isolate SRJPs from RJ. This new method produces 20-fold higher levels of SRJPs than that of the conventional procedure; hence, the levels obtained by the new and existing methods were compared. A 1-h ultracentrifugation separated SRJPs in the supernatant into upper, middle and lower layers. Each layer was analyzed by size-exclusion HPLC, SDS–PAGE and 2-DE. The upper and middle layers contained MRJP2 (52 kDa) and MRJP3 (60–70 kDa), while the lower layer contained MRJP1 (290 kDa). In nature, MRJP1 is a monomer and/or oligomer. When the lower layer was analyzed by Superose 12 HPLC, MRJP1 was predominantly an oligomer. Our MRJP isolation method reduces the procedure time by using ultracentrifugation without dialysis to obtain SRJPs and produces layers containing MRJP1 oligomers, MRJP2 and MRJP3. | |||||
| 言語 | en | |||||
| 注記 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Author | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| ID(XooNIps) | ||||||
| 値 | FC134-4-2332 | |||||
| 閲覧数(XooNIps) | ||||||
| 値 | 1111 | |||||
| ダウンロード数(XooNIps) | ||||||
| 値 | 1332 | |||||
