| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2010-02-01 |
| タイトル |
|
|
タイトル |
Effects of antidepressants on GluR2 Q/R site-RNA editing in modified HeLa cell line. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
相澤, 仁志
Sawada, J
Yamashita, T
Aburakawa, Y
Hasebe, N
Kwak, S
|
| 著者 ローマ字 |
|
|
|
Aizawa, Hitoshi |
| 著者 ローマ字 |
|
|
|
en |
| 書誌情報 |
Neuroscience Research
巻 64,
号 3,
p. 251-258,
発行日 2009-07-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0168-0102 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.neures.2009.03.009 |
| リンクURL |
|
|
内容記述タイプ |
Other |
|
内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Effects%20of%20antidepressants%20on%20GluR2%20Q%2FR%20site-RNA%20editing%20in%20modified%20HeLa%20cell%20line. | http://www.ncbi.nlm.nih.gov/pubmed?term=Effects%20of%20antidepressants%20on%20GluR2%20Q%2FR%20site-RNA%20editing%20in%20modified%20HeLa%20cell%20line. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Marked reduction of RNA editing at the glutamine (Q)/arginine (R) site of the glutamate receptor subunit type 2 (GluR2) in motor neurons may be a contributory cause of neuronal death specifically in sporadic ALS. It has been shown that deregulation of RNA editing of several mRNAs plays a causative role in diseases of the central nervous system such as depression. We analyzed the effects of eight antidepressants on GluR2 Q/R site-RNA editing in a modified HeLa cell line that stably expresses half-edited GluR2 pre-mRNA. We also measured changes in RNA expression levels of adenosine deaminase acting on RNA type 2 (ADAR2), the specific RNA editing enzyme of the GluR2 Q/R site, and GluR2, in order to assess the molecular mechanism causing alteration of this site-editing. The editing efficiency at the GluR2 Q/R site was significantly increased after treatment with seven out of eight antidepressants at a concentration of no more than 10 μM for 24 h. The relative abundance of ADAR2 mRNA to GluR2 pre-mRNA or to β-actin mRNA was increased after treatment with six of the effective antidepressants, whereas it was unchanged after treatment with milnacipran. Our results suggest that antidepressants have the potency to enhance GluR2 Q/R site-editing by either upregulating the ADAR2 mRNA expression level or other unidentified mechanisms. It may be worth investigating the in vivo efficacy of antidepressants with a specific therapeutic strategy for sporadic ALS in view. |
|
言語 |
en |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
|
|
出版タイプ |
AM |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
|
|
|
19447293 |
| 閲覧数(XooNIps) |
|
| ダウンロード数(XooNIps) |
|
|
|
2181 |
2828.pdf (434.5 kB)
