| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2009-06-16 |
| タイトル |
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|
タイトル |
Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
本間, 大
Stubbs, M
Aherne, W
Workman, P
Watt, FM
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| 著者 ローマ字 |
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|
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Honma, Masaru |
| 著者 ローマ字 |
|
|
|
en |
| 書誌情報 |
Journal of biomolecular screening
巻 11,
号 8,
p. 977-984,
発行日 2006-01-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1087-0571 |
| DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1177/1087057106292556 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed | http://www.ncbi.nlm.nih.gov/pubmed |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
We have attempted a high throughput-screening to find differentiation modifying compounds of keratinocyte. 11 inhibitors of known signaling pathway and approximately 4000 chemical compounds were screened using an In Cell Western system based on immunofluorescent staining. Staurosporine, a protein kinase C inhibitor and H89, a protein kinase A inhibitor, promote the expression of involucrin, a terminal differentiation marker in keratinocytes. On the other hand, U0126, a MEK1 specific inhibitor, and SAHA or SBHA, two HDAC inhibitors, prevent the expression of involucrin during calcium induced differentiation. In addition, we have found a novel compound that induces differentiation and two compounds that inhibit the differentiation of keratinocytes. One of these compounds also induced differentiation of a SCC cell line as well as apoptosis with a lower IC50 than that towards primary keratinocyte. These compounds might be useful in the future as tools to study differentiation of stratified epithelium as well as putative anti-tumor targets. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
‘The final, definitive version of this paper has been published in Journal of biomolecular screening, 11/8, Dec/2006 SAGE Publications Ltd., All rights reserved, 2006 by SAGE Publications Ltd at the Journal of biomolecular screening page: http://jbx.sagepub.com/ on SAGE Journals Online: http://online.sagepub.com/
\nauthor |
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言語 |
en |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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17092913 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1248 |
2218.pdf (3.0 MB)
