| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2009-05-15 |
| タイトル |
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タイトル |
Expression and secretion of cathelicidin antimicrobial peptides in murine mammary glands and human milk |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
村上, 正基
Stern, LJ
Lin, KH
Dorshner, RA
Gallo, RL
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| 著者 ローマ字 |
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Murakami, Masamoto |
| 著者 ローマ字 |
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en |
| 書誌情報 |
Pediatric research
巻 57,
号 1,
p. 10-15,
発行日 2005-01-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0031-3998 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1203/01.PDR.0000148068.32201.50 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.pedresearch.org/pt/re/pedresearch/home.htm;jsessionid=JvJh0yD2p574QQzRqJZcJ7PmLb4GhFF1wYyk89TwXJ5y3kK5TjqJ!-707522149!181195629!8091!-1 | http://www.pedresearch.org/pt/re/pedresearch/home.htm;jsessionid=JvJh0yD2p574QQzRqJZcJ7PmLb4GhFF1wYyk89TwXJ5y3kK5TjqJ!-707522149!181195629!8091!-1 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Mammalian milk possesses inherent antimicrobial properties that have been attributed to several diverse molecules. Recently, antimicrobial peptides that belong to the cathelicidin gene family have been found to be important to the mammalian immune response. This antimicrobial is expressed in several tissues and increased in neonatal skin, possibly to compensate for an immature adaptive immune response. We hypothesized that the mammary gland could produce and secrete cathelicidin onto the epithelial surface and into milk. Human cathelicidin hCAP18/LL-37 mRNA was detected in human milk cells by PCR. Quantitative real-time PCR demonstrated an increase in relative expression levels at 30 and 60 d after parturition. Immunohistochemistry of mouse breast tissue identified the murine cathelicidin-related antimicrobial peptide in lobuloacinar and ductules. Western blot analysis of human milk showed that LL-37 was secreted and present in the mature peptide form. The antimicrobial activity of LL-37 against Staphylococcus aureus, group A Streptococcus, and enteroinvasive Escherichia coli O29 in the human milk ionic environment was confirmed by solution colony-forming assay using synthetic peptide. These results indicate that cathelicidin is secreted in mammary gland and human milk, has antimicrobial activity against both Gram-positive and Gram-negative bacteria, and can contribute to the anti-infectious properties of milk. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
This is a non-final version of an article published in final form in Shuke, MURAKAMI, MASAMOTO ; DORSCHNER, ROBERT A .; STERN, LAUREN J. ; LIN, KENNETH H. ; GALLO, RICHARD L., Expression and secretion of cathelicidin antimicrobial peptides in murine mammary glands and human milk,Pediatric research 57(1), 2005, pp. 10-15
\nauthor |
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言語 |
en |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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15531744 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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2080 |
2142.pdf (322.8 kB)
