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Chromosome aberrations in mouse embryos and fetuses produced by assisted reproductive technology
https://asahikawa-med.repo.nii.ac.jp/records/1340
https://asahikawa-med.repo.nii.ac.jp/records/1340346d9252-367a-44e9-aca3-af4d7fd06b2a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1625.pdf (411.0 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||
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| 公開日 | 2009-03-11 | |||||||||
| タイトル | ||||||||||
| タイトル | Chromosome aberrations in mouse embryos and fetuses produced by assisted reproductive technology | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
立野, 裕幸
× 立野, 裕幸
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| 著者 ローマ字 | ||||||||||
| Tateno, Hiroyuki | ||||||||||
| 書誌情報 |
Mutation Research : Genetic Toxicology and Environmental Mutagenesis 巻 657, 号 1, p. 26-31, 発行日 2008-11-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1383-5718 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1016/j.mrgentox.2008.09.002 | |||||||||
| リンクURL | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | http://dx.doi.org/10.1016/j.mrgentox.2008.09.002 | http://dx.doi.org/10.1016/j.mrgentox.2008.09.002 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The occurrence of structural chromosome aberrations in mouse one-cell embryos produced by intracytoplasmic sperm injection (ICSI) with mature spermatozoa was dependent on the type of sperm incubation medium and sperm incubation time. When cauda epididymal spermatozoa were used following incubation in bicarbonate-buffered TYH medium for 0 h (no incubation) and 0.5 h, the chromosome aberration rates (6.9% and 7.4%, respectively) in the resultant embryos were significantly higher than that (2.3%) in the IVF embryos. However, when the spermatozoa were incubated for 2−2.5 h and 6 h in the same medium, the chromosome aberration rates were reduced to the IVF embryo level (3.8% and 4.3%, respectively). When spermatozoa incubated in Hepes-buffered H-mCZB and phosphate-buffered PB1 media were used for ICSI, chromosome aberration rates in embryos were significantly high (8.6−28.1%) and increased in a time-dependent manner. On the other hand, when immature testicular spermatozoa were incubated in those three media for 0.5 h and 6 h, the incidences of resultant embryos with structural chromosome aberrations ranged between 7.4% and 11.7%, and there was no medium- and time-dependent change in these aberration rates.To evaluate transmissible risk of chromosome aberrations to offspring, two- or four-cell embryos derived from cauda epididymal spermatozoa were transferred into the oviducts of pseudopregnant females and chromosomes of live fetuses were examined on gestational day 16. One (2.0%) mosaic fetus was found when spermatozoa were incubated in TYH for 2−2.5 h, and there were four (6.7%) fetuses displaying a structurally abnormal karyotype when spermatozoa were incubated in H-mCZB for 2−2.5 h, indicating that structural chromosome aberrations generated in ICSI one-cell embryos are transmissible to offspring. The causal mechanism of structural chromosome aberrations in ICSI one-cell embryos is discussed in relation to the acrosomal plasma membrane cholesterol and the acrosome. | |||||||||
| 注記 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 注記 | author | |||||||||
| 資源タイプ | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 資源タイプ | text | |||||||||
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| 出版タイプ | AM | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| ID(XooNIps) | ||||||||||
| 18852067 | ||||||||||
| 閲覧数(XooNIps) | ||||||||||
| ダウンロード数(XooNIps) | ||||||||||
| 938 | ||||||||||
