{"created":"2023-06-19T11:20:38.668857+00:00","id":707,"links":{},"metadata":{"_buckets":{"deposit":"c1c37017-13dd-425e-8cc4-f47f07436e11"},"_deposit":{"created_by":3,"id":"707","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"707"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000707","sets":["7","7:24"]},"author_link":["1934","1935","1936"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageEnd":"13619","bibliographicPageStart":"13615","bibliographicVolumeNumber":"277","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.ncbi.nlm.nih.gov/pubmed?term=Remarkable%20stability%20of%20solubilized%20and%20delipidated%20sarcoplasmic%20reticulum%20Ca2%2B-ATPase%20with%20tightly%20bound%20fluoride%20and%20magnesium%20against%20detergent-induced%20denaturation | http://www.ncbi.nlm.nih.gov/pubmed?term=Remarkable%20stability%20of%20solubilized%20and%20delipidated%20sarcoplasmic%20reticulum%20Ca2%2B-ATPase%20with%20tightly%20bound%20fluoride%20and%20magnesium%20against%20detergent-induced%20denaturation","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Conditions were developed in the absence of Ca(2+) for purification, delipidation, and long term stabilization of octaethylene glycol monododecyl ether (C(12)E(8))-solubilized sarcoplasmic reticulum Ca(2+)-ATPase with tightly bound Mg(2+) and F(-), an analog for the phosphoenzyme intermediate without bound Ca(2+). The Ca(2+)-ATPase activity to monitor denaturation was assessed after treatment with 20 mm Ca(2+) to release tightly bound Mg(2+)/F(-). The purification and delipidation was successfully achieved with Reactive Red-agarose affinity chromatography. The solubilized Mg(2+)/F(-)-bound Ca(2+)-ATPase was very rapidly denatured at pH 8, but was perfectly stabilized at pH 6 against denaturation for over 20 days at 4 degrees C even without exogenously added phospholipid and at a high C(12)E(8)/enzyme weight ratio (10:1). The activity was not restored unless the enzyme was treated with 20 mm Ca(2+), showing that tightly bound Mg(2+)/F(-) was not released during the long term incubation. The perfect stability was attained with or without 0.1 mm dithiothreitol, but inactivation occurred with a half-life of 10 days in the presence of 1 mm dithiothreitol, possibly due to reduction of a specific disulfide bond(s). The remarkable stability is likely conferred by intimate gathering of cytoplasmic domains of Ca(2+)-ATPase molecule induced by tight binding of Mg(2+)/F(-). The present study thus reveals an essential property of the Mg(2+)/F(-)/Ca(2+)-ATPase complex, which will likely provide clues to understanding structure of the Ca(2+)-released form of phosphoenzyme intermediate at an atomic level.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"American Society for Biochemistry and Molecular Biology, Yamasaki, Kazuo ; Daiho, Takashi ; Suzuki, Hiroshi, Journal of Biological Chemistry, 277(16), 2002, 13615-13619\n\\nauthor","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M200625200","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"11830596"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2147"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"648"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Yamasaki, Kazuo"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 和生"},{"creatorName":"ヤマサキ, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1934","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daiho, T"}],"nameIdentifiers":[{"nameIdentifier":"1935","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, H"}],"nameIdentifiers":[{"nameIdentifier":"1936","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"887.pdf","filesize":[{"value":"344.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"887.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/707/files/887.pdf"},"version_id":"c7ff5532-4467-4fae-b005-eb9a866bf72b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-03-28"},"publish_date":"2008-03-28","publish_status":"0","recid":"707","relation_version_is_last":true,"title":["Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:24:22.395561+00:00"}