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Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation
https://asahikawa-med.repo.nii.ac.jp/records/707
https://asahikawa-med.repo.nii.ac.jp/records/7076ac995b0-85f6-484a-9e4a-9a238ada633f
| 名前 / ファイル | ライセンス | アクション |
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887.pdf (344.5 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||
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| 公開日 | 2008-03-28 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca2+-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
山崎, 和生
× 山崎, 和生
× Daiho, T
× Suzuki, H
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| 著者 ローマ字 | ||||||||||||||
| Yamasaki, Kazuo | ||||||||||||||
| 書誌情報 |
Journal of Biological Chemistry 巻 277, 号 16, p. 13615-13619, 発行日 2002-04-01 |
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0021-9258 | |||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1074/jbc.M200625200 | |||||||||||||
| リンクURL | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | http://www.ncbi.nlm.nih.gov/pubmed?term=Remarkable%20stability%20of%20solubilized%20and%20delipidated%20sarcoplasmic%20reticulum%20Ca2%2B-ATPase%20with%20tightly%20bound%20fluoride%20and%20magnesium%20against%20detergent-induced%20denaturation | http://www.ncbi.nlm.nih.gov/pubmed?term=Remarkable%20stability%20of%20solubilized%20and%20delipidated%20sarcoplasmic%20reticulum%20Ca2%2B-ATPase%20with%20tightly%20bound%20fluoride%20and%20magnesium%20against%20detergent-induced%20denaturation | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Conditions were developed in the absence of Ca(2+) for purification, delipidation, and long term stabilization of octaethylene glycol monododecyl ether (C(12)E(8))-solubilized sarcoplasmic reticulum Ca(2+)-ATPase with tightly bound Mg(2+) and F(-), an analog for the phosphoenzyme intermediate without bound Ca(2+). The Ca(2+)-ATPase activity to monitor denaturation was assessed after treatment with 20 mm Ca(2+) to release tightly bound Mg(2+)/F(-). The purification and delipidation was successfully achieved with Reactive Red-agarose affinity chromatography. The solubilized Mg(2+)/F(-)-bound Ca(2+)-ATPase was very rapidly denatured at pH 8, but was perfectly stabilized at pH 6 against denaturation for over 20 days at 4 degrees C even without exogenously added phospholipid and at a high C(12)E(8)/enzyme weight ratio (10:1). The activity was not restored unless the enzyme was treated with 20 mm Ca(2+), showing that tightly bound Mg(2+)/F(-) was not released during the long term incubation. The perfect stability was attained with or without 0.1 mm dithiothreitol, but inactivation occurred with a half-life of 10 days in the presence of 1 mm dithiothreitol, possibly due to reduction of a specific disulfide bond(s). The remarkable stability is likely conferred by intimate gathering of cytoplasmic domains of Ca(2+)-ATPase molecule induced by tight binding of Mg(2+)/F(-). The present study thus reveals an essential property of the Mg(2+)/F(-)/Ca(2+)-ATPase complex, which will likely provide clues to understanding structure of the Ca(2+)-released form of phosphoenzyme intermediate at an atomic level. | |||||||||||||
| 注記 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 注記 | American Society for Biochemistry and Molecular Biology, Yamasaki, Kazuo ; Daiho, Takashi ; Suzuki, Hiroshi, Journal of Biological Chemistry, 277(16), 2002, 13615-13619 \nauthor |
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| 内容記述タイプ | Other | |||||||||||||
| 資源タイプ | text | |||||||||||||
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| 出版タイプ | AM | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
| ID(XooNIps) | ||||||||||||||
| 11830596 | ||||||||||||||
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| ダウンロード数(XooNIps) | ||||||||||||||
| 648 | ||||||||||||||
