{"created":"2023-06-19T11:20:38.077173+00:00","id":695,"links":{},"metadata":{"_buckets":{"deposit":"6d682870-17f8-4c9b-b4f6-28bf8bcbcf03"},"_deposit":{"created_by":3,"id":"695","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"695"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000695","sets":["7","7:24"]},"author_link":["1864","1865","1866","1867"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"923","bibliographicPageStart":"920","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Antimicrobial Agents and Chemotherapy"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.ncbi.nlm.nih.gov/pubmed?term=Analysis%20of%20Phosphorylation%20Pathways%20of%20Antiherpesvirus%20Nucleosides%20by%20Varicella-Zoster%20Virus-Specific%20Enzymes | http://www.ncbi.nlm.nih.gov/pubmed?term=Analysis%20of%20Phosphorylation%20Pathways%20of%20Antiherpesvirus%20Nucleosides%20by%20Varicella-Zoster%20Virus-Specific%20Enzymes","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The inhibitory activities of acyclovir (ACV), 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil (BV-araU), ganciclovir (GCV), 9-(2-deoxy-2-hydroxymethyl-beta-D-erythro-oxetanosyl)guanine (OXT-G), and (+)-9-[(1R,2R,3S)-2,3-bis(hydroxymethyl)Cyclobutyl]guanine (cOXT-G) on the replication of wild-type and thymidine kinase (TK)-negative strains of herpes simplex virus types 1 and 2 and varicella-zoster virus (VZV) and the wild-type strain of human cytomegalovirus were tested to clarity whether the phosphorylation of these compounds is catalyzed by viral TK or other enzymes. ACV and BV-araU had little effect on the replication of TK-negative virus strains. On the other hand, GCV, OXT-G, and cOXT-G inhibited the replication of TK-negative VZV at concentrations 10 times higher than those at which they inhibited wild-type VZV, indicating that a kinase other than TK phosphorylates GCV and OXT-G in VZV-infected cells. GCV phosphorylation activity was not detected in VZV-infected cell lysates; therefore, this activity was evaluated in COS 1 cells expressing viral TK and viral protein kinase (PK). The COS 1 cells expressing VZV TK were shown to be susceptible to all compounds tested. In contrast, VZV Pk-expressing COS 1 cells were susceptible to only GCV, OXT-G, and cOXT-G. These results suggest that VZV PK phosphorylates some nucleoside analogs, for example, GCV, OXT-G, and cOXT-G. This phosphorylation pathway may be important in the anti-VZV activities of some nucleoside analogs.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"Copyright © American Society for Microbiology, Antimicrobial Agents and Chemotherapy, volume 40, 920-923, 1996\n\\npublisher","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0066-4804","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"8849252"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"3225"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2819"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Koyano, Shin"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"古谷野, 伸"},{"creatorName":"コヤノ, シン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1864","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzutani, T"}],"nameIdentifiers":[{"nameIdentifier":"1865","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, A"}],"nameIdentifiers":[{"nameIdentifier":"1866","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Azuma, M"}],"nameIdentifiers":[{"nameIdentifier":"1867","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"870.pdf","filesize":[{"value":"237.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"870.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/695/files/870.pdf"},"version_id":"39c5397b-c95b-4cc0-91c5-aa843ecd621b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analysis of Phosphorylation Pathways of Antiherpesvirus Nucleosides by Varicella-Zoster Virus-Specific Enzymes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of Phosphorylation Pathways of Antiherpesvirus Nucleosides by Varicella-Zoster Virus-Specific Enzymes","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-28"},"publish_date":"2008-02-28","publish_status":"0","recid":"695","relation_version_is_last":true,"title":["Analysis of Phosphorylation Pathways of Antiherpesvirus Nucleosides by Varicella-Zoster Virus-Specific Enzymes"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:28:26.765620+00:00"}