| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-02-28 |
| タイトル |
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タイトル |
Analysis of Phosphorylation Pathways of Antiherpesvirus Nucleosides by Varicella-Zoster Virus-Specific Enzymes |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
古谷野, 伸
Suzutani, T
Yoshida, A
Azuma, M
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| 著者 ローマ字 |
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Koyano, Shin |
| 書誌情報 |
Antimicrobial Agents and Chemotherapy
巻 40,
号 4,
p. 920-923,
発行日 1996-01-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0066-4804 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Analysis%20of%20Phosphorylation%20Pathways%20of%20Antiherpesvirus%20Nucleosides%20by%20Varicella-Zoster%20Virus-Specific%20Enzymes | http://www.ncbi.nlm.nih.gov/pubmed?term=Analysis%20of%20Phosphorylation%20Pathways%20of%20Antiherpesvirus%20Nucleosides%20by%20Varicella-Zoster%20Virus-Specific%20Enzymes |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The inhibitory activities of acyclovir (ACV), 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil (BV-araU), ganciclovir (GCV), 9-(2-deoxy-2-hydroxymethyl-beta-D-erythro-oxetanosyl)guanine (OXT-G), and (+)-9-[(1R,2R,3S)-2,3-bis(hydroxymethyl)Cyclobutyl]guanine (cOXT-G) on the replication of wild-type and thymidine kinase (TK)-negative strains of herpes simplex virus types 1 and 2 and varicella-zoster virus (VZV) and the wild-type strain of human cytomegalovirus were tested to clarity whether the phosphorylation of these compounds is catalyzed by viral TK or other enzymes. ACV and BV-araU had little effect on the replication of TK-negative virus strains. On the other hand, GCV, OXT-G, and cOXT-G inhibited the replication of TK-negative VZV at concentrations 10 times higher than those at which they inhibited wild-type VZV, indicating that a kinase other than TK phosphorylates GCV and OXT-G in VZV-infected cells. GCV phosphorylation activity was not detected in VZV-infected cell lysates; therefore, this activity was evaluated in COS 1 cells expressing viral TK and viral protein kinase (PK). The COS 1 cells expressing VZV TK were shown to be susceptible to all compounds tested. In contrast, VZV Pk-expressing COS 1 cells were susceptible to only GCV, OXT-G, and cOXT-G. These results suggest that VZV PK phosphorylates some nucleoside analogs, for example, GCV, OXT-G, and cOXT-G. This phosphorylation pathway may be important in the anti-VZV activities of some nucleoside analogs. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright © American Society for Microbiology, Antimicrobial Agents and Chemotherapy, volume 40, 920-923, 1996
\npublisher |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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8849252 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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2819 |
870.pdf (237.9 kB)
