{"created":"2023-06-19T11:20:37.879216+00:00","id":691,"links":{},"metadata":{"_buckets":{"deposit":"873c6ac7-410d-489d-9c3a-f4d1a3dcfd03"},"_deposit":{"created_by":3,"id":"691","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"691"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000691","sets":["7","7:24"]},"author_link":["1837","1838","1839","1840","1841","1842","1843","1844","1845","1846","1847","1848"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"1088","bibliographicPageStart":"1082","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Journal of Clinical Microbiology."}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.ncbi.nlm.nih.gov/pubmed?term=Molecular%20cloning%2C%20expression%2C%20and%20serological%20evaluation%20of%20an%208-kilodalton%20subunit%20of%20antigen%20B%20from%20Echinococcus%20multilocularis. | http://www.ncbi.nlm.nih.gov/pubmed?term=Molecular%20cloning%2C%20expression%2C%20and%20serological%20evaluation%20of%20an%208-kilodalton%20subunit%20of%20antigen%20B%20from%20Echinococcus%20multilocularis.","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Full-length cDNA and genomic DNA encoding an 8-kDa subunit of antigen B from Echinococcus multilocularis (designated EmAgB8/1) were isolated from an E. multilocularis metacestode cDNA library and a protoscolex genomic DNA library, respectively. The open reading frame of the cDNA clone encodes a polypeptide comprising 85 amino acids with a 20-amino-acid NH(2)-terminal signal sequence, which was confirmed following N-terminal sequencing of the native antigen. Reverse transcription-PCR analysis revealed that the clone encoding EmAgB8/1 is predominantly transcribed in larval E. multilocularis. The gene consists of two exons (encoding the signal sequence and mature protein) separated by a 91-bp intron. The mature form was expressed in Escherichia coli, and its antigenic reactivity was compared with that of a counterpart, an 8-kDa subunit of antigen B from Echinococcus granulosus (EgAgB8/1) by Western blotting and enzyme-linked immunosorbent assay (ELISA) with serum samples from patients confirmed to have cystic echinococcosis (CE) and alveolar echinococcosis (AE). Recombinant EmAgB8/1 showed positive reactions in Western blots with 81.3% (65 of 80) of serum samples from CE patients and 40.6% (26 of 64) of serum samples from AE patients, while recombinant EgAgB8/1 showed positive reactions with 86% (43 of 50) and 42% (19 of 45) of the serum samples from these CE and AE patients, respectively. By the ELISA, both EmAgB8/1 and EgAgB8/1 exhibited similar positive reactions with 88% (44 of 50) of serum samples from CE patients and 37.8% (17 of 45) serum samples from AE patients. Statistical analysis revealed that the sensitivity of EmAgB8/1 was comparable to that of EgAgB8/1 for the serodiagnosis of echinococcal diseases. There was no cross-reaction with sera from patients with cysticercosis, which often cross-react when native antigens are used for serodiagnosis.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"Copyright © American Society for Microbiology, Journal of Clinical Microbiology, volume 42, 1082-1088, 2004\n\\npublisher","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/JCM.42.3.1082-1088.2004","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0095-1137","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"15004057"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2285"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1541"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Ito, Akira"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"伊藤, 亮"},{"creatorName":"イトウ, アキラ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mamuti, W"}],"nameIdentifiers":[{"nameIdentifier":"1838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamasaki, H"}],"nameIdentifiers":[{"nameIdentifier":"1839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sako, Y"}],"nameIdentifiers":[{"nameIdentifier":"1840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakao, M"}],"nameIdentifiers":[{"nameIdentifier":"1841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Xiao, N"}],"nameIdentifiers":[{"nameIdentifier":"1842","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakaya, K"}],"nameIdentifiers":[{"nameIdentifier":"1843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, N"}],"nameIdentifiers":[{"nameIdentifier":"1844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vuitton, DA"}],"nameIdentifiers":[{"nameIdentifier":"1845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Piarroux, R"}],"nameIdentifiers":[{"nameIdentifier":"1846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lightowlers, MW"}],"nameIdentifiers":[{"nameIdentifier":"1847","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Craig, PS"}],"nameIdentifiers":[{"nameIdentifier":"1848","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"866.pdf","filesize":[{"value":"390.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"866.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/691/files/866.pdf"},"version_id":"b03bf7d0-5c49-4e35-b83f-7eb0c018b39a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-28"},"publish_date":"2008-02-28","publish_status":"0","recid":"691","relation_version_is_last":true,"title":["Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis."],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:28:42.249167+00:00"}