| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-02-28 |
| タイトル |
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タイトル |
Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
伊藤, 亮
Mamuti, W
Yamasaki, H
Sako, Y
Nakao, M
Xiao, N
Nakaya, K
Sato, N
Vuitton, DA
Piarroux, R
Lightowlers, MW
Craig, PS
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| 著者 ローマ字 |
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Ito, Akira |
| 書誌情報 |
Journal of Clinical Microbiology.
巻 42,
号 3,
p. 1082-1088,
発行日 2004-03-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0095-1137 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JCM.42.3.1082-1088.2004 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Molecular%20cloning%2C%20expression%2C%20and%20serological%20evaluation%20of%20an%208-kilodalton%20subunit%20of%20antigen%20B%20from%20Echinococcus%20multilocularis. | http://www.ncbi.nlm.nih.gov/pubmed?term=Molecular%20cloning%2C%20expression%2C%20and%20serological%20evaluation%20of%20an%208-kilodalton%20subunit%20of%20antigen%20B%20from%20Echinococcus%20multilocularis. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Full-length cDNA and genomic DNA encoding an 8-kDa subunit of antigen B from Echinococcus multilocularis (designated EmAgB8/1) were isolated from an E. multilocularis metacestode cDNA library and a protoscolex genomic DNA library, respectively. The open reading frame of the cDNA clone encodes a polypeptide comprising 85 amino acids with a 20-amino-acid NH(2)-terminal signal sequence, which was confirmed following N-terminal sequencing of the native antigen. Reverse transcription-PCR analysis revealed that the clone encoding EmAgB8/1 is predominantly transcribed in larval E. multilocularis. The gene consists of two exons (encoding the signal sequence and mature protein) separated by a 91-bp intron. The mature form was expressed in Escherichia coli, and its antigenic reactivity was compared with that of a counterpart, an 8-kDa subunit of antigen B from Echinococcus granulosus (EgAgB8/1) by Western blotting and enzyme-linked immunosorbent assay (ELISA) with serum samples from patients confirmed to have cystic echinococcosis (CE) and alveolar echinococcosis (AE). Recombinant EmAgB8/1 showed positive reactions in Western blots with 81.3% (65 of 80) of serum samples from CE patients and 40.6% (26 of 64) of serum samples from AE patients, while recombinant EgAgB8/1 showed positive reactions with 86% (43 of 50) and 42% (19 of 45) of the serum samples from these CE and AE patients, respectively. By the ELISA, both EmAgB8/1 and EgAgB8/1 exhibited similar positive reactions with 88% (44 of 50) of serum samples from CE patients and 37.8% (17 of 45) serum samples from AE patients. Statistical analysis revealed that the sensitivity of EmAgB8/1 was comparable to that of EgAgB8/1 for the serodiagnosis of echinococcal diseases. There was no cross-reaction with sera from patients with cysticercosis, which often cross-react when native antigens are used for serodiagnosis. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright © American Society for Microbiology, Journal of Clinical Microbiology, volume 42, 1082-1088, 2004
\npublisher |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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15004057 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1541 |
866.pdf (390.3 kB)
