| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-03-22 |
| タイトル |
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タイトル |
Carrier-mediated processes in blood-brain barrier penetration and neural uptake of paraquat |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
清水, 恵子
Ohtaki, K
Matsubara, K
Aoyama, K
Uezono, T
Saito, O
Suno, M
Ogawa, K
Hayase, N
Kimura, K
Shiono, H
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| 著者 ローマ字 |
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Shimizu, Keiko |
| 書誌情報 |
BRAIN RESEARCH
巻 906,
号 1-2,
p. 135-142,
発行日 2001-07-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-8993 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/S0006-8993(01)02577-X |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.sciencedirect.com/science/journal/00068993 | http://www.sciencedirect.com/science/journal/00068993 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Due to the structural similarity to N-methyl-4-phenyl pyridinium (MPP+), paraquat might induce dopaminergic toxicity in the brain. However, its blood-brain barrier (BBB) penetration has not been well documented. We studied the manner of BBB penetration and neural cell uptake of paraquat using a brain microdialysis technique with the HPLC/UV detection in rats. After subcutaneous administration, paraquat appeared dose-dependently in the dialysate. In contrast, MPP+ could not penetrate the BBB in either control or paraquat pre-treated rats. These data indicated that the penetration of paraquat into the brain would be mediated by a specific carrier process, not resulting from the destruction of the BBB function by paraquat itself or a paraquat radical. To examine whether paraquat was carried across the BBB by a certain amino acid transporter, L-valine or L-lysine was pre-administered as a co-substrate. The pre-treatment of L-valine, which is a high affinity substrate for the neutral amino acid transporter, markedly reduced the BBB penetration of paraquat. When paraquat was administered to the striatum through a microdialysis probe, a significant amount of paraquat was detected in the striatal cells after a sequential 180-min washout with Ringer's solution. This uptake was significantly inhibited by a low Na+ condition, but not by treatment with putrescine, a potent uptake inhibitor of paraquat into lung tissue. These findings indicated that paraquat is possibly taken up into the brain by the neutral amino acid transport system, then transported into striatal, possibly neuronal, cells in a Na+-dependent manner. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
ELSEVIER, Ishimoto, T; Omori, N; Mutoh, F; Chiba, S, BRAIN RESEARCH, 906(1-2), 152-158, 2001.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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11430870 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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3289 |
367.pdf (387.9 kB)
