| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-10-29 |
| タイトル |
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|
タイトル |
Denatured human alpha-defensin attenuates the bactericidal activity and the stability against enzymatic digestion |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
田邉, 裕貴
Ayabe, T
Maemoto, A
Ishikawa, C
Inabe, Y
Sato, R
Moriichi, K
Okamoto, K
Watari, J
Kono, T
Ashida, T
Kohgo, Y
|
| 著者 ローマ字 |
|
|
|
Tanabe, Hiroki |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 358,
号 1,
p. 349-355,
発行日 2007-01-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| DOI |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.bbrc.2007.04.132 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://www.sciencedirect.com/science/journal/0006291X | http://www.sciencedirect.com/science/journal/0006291X |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
α-Defensin is an antimicrobial peptide which plays an important role in innate immunity. Human defensin (HD)-5 is stored in the Paneth cells of the small intestine as a pro-form and is cleaved by trypsin, which is co-secreted from the Paneth cell granules. The mature HD-5 is protected from further digestion by the proteolysis enzyme. We generated both recombinant HD-5 and proHD-5, and the reduced form of each peptide in order to determine their physiological roles of the disulfide bonds. The reduced proHD-5 attenuated the bactericidal activity and the stability against the trypsin digestion. Human defensin was protected from the enzymatic degradation by disulfide bridges. We further purified the HD-5 with a disulfide variation in the small intestine of Crohn’s disease patients. The HD-5 was sensitive to the trypsin treatment. These observations evidently predict that a defensin deficiency may be caused by a disulfide disorder in the disease. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Elsevier, Tanabe, Hiroki ; Ayabe, Tokiyoshi ; Maemoto, Atsuo ; Ishikawa, Chisato ; Inaba, Yuhei ; Sato, Ryu ; Moriichi, Kentaro ; Okamoto, Kotaro ; Watari, Jiro ; Kono, Toru ; Ashida, Toshifumi ; Kohgo, Yutaka, Biochemical and Biophysical Research Communications, 358(1), 2007, 349-335.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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17482139 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1499 |
773.pdf (4.3 MB)
