{"created":"2023-06-19T11:25:13.993596+00:00","id":6061,"links":{},"metadata":{"_buckets":{"deposit":"d0fefae7-3a48-4c67-9dac-9cf1c16611ca"},"_deposit":{"created_by":3,"id":"6061","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6061"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00006061","sets":["7","7:27:41"]},"author_link":["19075"],"item_10_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Mir-30eは細胞外小胞EVによる細胞間伝達を介し上皮間葉形質転換EMTの抑制を経て胆管癌細胞の浸潤・遊走能を阻害する"}]},"item_10_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23","bibliographicPageEnd":"16417","bibliographicPageStart":"16400","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Oncotarget"}]}]},"item_10_degree_grantor_32":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"旭川医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10107","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID：29662654","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Early-staged cholangiocarcinoma (CCA) is difficult to diagnose due to its high potential for invasion and metastasis. Epithelial-mesenchymal transition (EMT) is induced by transforming growth factor-β (TGF-β) in a process thought to be important for invasion and metastasis in several cancers, including CCA. Although microRNAs (miRNAs) have been implicated in the pathogenesis of several malignancies, their roles to CCA are not clearly understood. Some miRNAs were reported to be included in extracellular vesicles (EVs) and transferred from their donor cells to other cells, modulating recipient cell behaviors. In this study, the involvement and functional roles of EV-contained miRNAs during EMT in human CCA were determined. Expression profiling identified a subset of miRNAs that were reduced by TGF-β in CCA cells. Among these, miR-30e was highly downregulated by TGF-β and predicted to target Snail, which is an EMT-inducible transcription factor. MiR-30e overexpression suppressed cell invasion and migration via inhibiting EMT, whereas miR-30e inhibition promoted EMT, cell invasion and migration. Moreover, miR-30e was enriched in EVs derived from CCA cells after miR-30e overexpression, and miR-30e intercellular transfer through EVs suppressed EMT, cell invasion and migration in recipient CCA cells. Together, our results suggest that EV-mediated miR-30e transfer could inhibit EMT via directly targeting Snail, which subsequently suppresses CCA cell invasion and migration. These findings provide several new insights into regulatory mechanisms of tumor invasion and metastasis in human CCA.","subitem_description_type":"Abstract"}]},"item_10_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_dissertation_number_29":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲532"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.18632/oncotarget.24711","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1949-2553","subitem_source_identifier_type":"ISSN"}]},"item_10_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Ota, Yu"}]},"item_10_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"太田, 雄","creatorNameLang":"ja"},{"creatorName":"オオタ, ユウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"19075","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"7214.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"7214.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/6061/files/7214.pdf"},"version_id":"6422d970-8398-4251-a581-6ad1d298087c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cholangiocarcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"epithelial-mesenchymal transition (EMT)","subitem_subject_scheme":"Other"},{"subitem_subject":"exosome","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular vesicles","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA (miRNA)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Extracellular vesicle-encapsulated miR-30e suppresses　cholangiocarcinoma cell invasion and migration via inhibiting epithelial-mesenchymal transition","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Extracellular vesicle-encapsulated miR-30e suppresses　cholangiocarcinoma cell invasion and migration via inhibiting epithelial-mesenchymal transition","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["6","7","41"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-26"},"publish_date":"2019-03-26","publish_status":"0","recid":"6061","relation_version_is_last":true,"title":["Extracellular vesicle-encapsulated miR-30e suppresses　cholangiocarcinoma cell invasion and migration via inhibiting epithelial-mesenchymal transition"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-09T06:59:30.868432+00:00"}