{"created":"2023-06-19T11:25:05.766954+00:00","id":5913,"links":{},"metadata":{"_buckets":{"deposit":"0cec7d4e-01c0-4129-b2ed-f4e9de430753"},"_deposit":{"created_by":3,"id":"5913","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5913"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005913","sets":["7","7:27:41"]},"author_link":["18545"],"item_10_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"血清エクソソーム中microRNA-191,-21,-451aは膵腫瘍の有用な診断マーカーとなり得る"}]},"item_10_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"116","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"BMC Cancer"}]}]},"item_10_degree_grantor_32":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"旭川医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10107","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID：29385987","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\n\\nPancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers.\nMETHODS:\n\\nThe ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction.\nRESULTS:\n\\nThe expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5-20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022).\nCONCLUSIONS:\n\\nThe level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs).","subitem_description_type":"Abstract"}]},"item_10_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_dissertation_number_29":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙473"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s12885-018-4006-5.","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1471-2407","subitem_source_identifier_type":"ISSN"}]},"item_10_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Goto, Takuma"}]},"item_10_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"後藤, 拓磨","creatorNameLang":"ja"},{"creatorName":"ゴトウ, タクマ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18545","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"7026.pdf","filesize":[{"value":"820.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"7026.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5913/files/7026.pdf"},"version_id":"943a0598-8ed2-4481-b332-f8b3c53bf828"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Exosome","subitem_subject_scheme":"Other"},{"subitem_subject":"Intraductal papillary mucinous neoplasm","subitem_subject_scheme":"Other"},{"subitem_subject":"Pancreatic cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"Tumor marker","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA-21","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA-451a","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["6","7","41"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-07-03"},"publish_date":"2018-07-03","publish_status":"0","recid":"5913","relation_version_is_last":true,"title":["An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker."],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-09T06:59:13.029545+00:00"}