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Role of the BrafV637E mutation in hepatocarcinogenesis induced by treatment with diethylnitrosamine in neonatal B6C3F1 mice.
https://asahikawa-med.repo.nii.ac.jp/records/5728
https://asahikawa-med.repo.nii.ac.jp/records/5728e2aa092d-7b33-4fb5-ad8f-a8c72fd21ec4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6794.pdf (2.5 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-09-20 | |||||
| タイトル | ||||||
| タイトル | Role of the BrafV637E mutation in hepatocarcinogenesis induced by treatment with diethylnitrosamine in neonatal B6C3F1 mice. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | BrafV600E transgenic mice | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cytokines/chemokines | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hepatic tumors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | intracellular signaling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | whole-exome analysis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
山本, 雅大
× 山本, 雅大× 田中, 宏樹× Bing, Xin× 西川, 祐司× 山崎, 弘資× 清水, 惠子× 小川, 勝洋 |
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| 著者 ローマ字 | ||||||
| 値 | Yamamoto, Masahiro | |||||
| 著者 ローマ字 | ||||||
| 値 | Tanaka, Hiroki | |||||
| 著者 ローマ字 | ||||||
| 著者 ローマ字 | ||||||
| 値 | Nishikawa, Yuji | |||||
| 著者 ローマ字 | ||||||
| 値 | Yamazaki, Kosuke | |||||
| 著者 ローマ字 | ||||||
| 値 | Shimizu, Keiko | |||||
| 著者 ローマ字 | ||||||
| 値 | Ogawa, Katsuhiro | |||||
| 書誌情報 |
Molecular carcinogenesis 巻 56, 号 2, p. 478-488, 発行日 2017-02-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0899-1987 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/mc.22510. | |||||
| リンクURL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://onlinelibrary.wiley.com/doi/10.1002/mc.22510/full/ | http://onlinelibrary.wiley.com/doi/10.1002/mc.22510/full/ | |||||
| 識別番号 その他 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PMID：27253992 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The BrafV637E mutation is frequently reported in mouse hepatic tumors, depending on the mouse strain, and corresponds to the human BrafV600E mutation. In this study, we detected the BrafV637E mutation by whole-exome analysis in 4/4 hepatic tumors induced by neonatal treatment with diethylnitrosamine (DEN) in male B6C3F1 mice. We also detected the BrafV637E mutation in 54/63 (85.7%) hepatic lesions, including microscopic foci and grossly visible tumors, by PCR-direct sequencing. Although the mutation was detected in 5/7 (71.4%) hepatic tumors induced by neonatal DEN treatment followed by repeated CCl4 administration, it was not detected in 24 tumors induced by CCl4 treatment without DEN or in eight spontaneous lesions in B6C3F1 mice, suggesting that the mutation is induced by the genotoxic action of DEN. The DEN-induced tumors exhibited hyperphosphorylation of ERK1 and Akt, suggesting that the BrafV637E mutation might activate the MAPK and Akt pathways. Moreover, the DEN-induced tumors overexpressed mRNAs for the oncogene-induced senescence (OIS) markers such as p15Ink4b and p19Arf as well as pro-survival/pro-proliferative cytokines/chemokines such as complement C5/C5a, ICAM-1, IL-1 receptor antagonist and CXCL9, suggesting that the BrafV637E mutation influences the expression of genes involved in either OIS or cellular growth/survival. Liver-specific expression of mutated Braf under control of the albumin enhancer/promoter resulted in an enlarged liver that consisted entirely of small basophilic hepatocytes resembling DEN-induced preneoplastic hepatocytes with ERK1/Akt hyperphosphorylation and C5/C5a overexpression. These results indicate that the BrafV637E mutation induces hepatocytic changes in DEN-induced hepatic tumors. © 2016 The Authors. Molecular Carcinogenesis published by Wiley Periodicals, Inc. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| ID(XooNIps) | ||||||
| 値 | 27253992 | |||||
| 閲覧数(XooNIps) | ||||||
| 値 | 428 | |||||
| ダウンロード数(XooNIps) | ||||||
| 値 | 792 | |||||
