{"created":"2023-06-19T11:24:55.001300+00:00","id":5706,"links":{},"metadata":{"_buckets":{"deposit":"111cc9f1-aa3b-4bb0-a073-62806efbb4e2"},"_deposit":{"created_by":1,"id":"5706","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5706"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005706","sets":["7","7:24"]},"author_link":["17832","17835","17836","17828","17834","17830","17840","17831","17833","17838","17839","17829","17826","17837","17827"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"507","bibliographicPageStart":"501","bibliographicVolumeNumber":"476","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_5_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID：27264950","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer including hepatoma. By RT-PCR/agarose gel electrophoresis of hepcidin mRNA in a hepatocellular carcinoma cell line HLF, a smaller mRNA band was shown in addition to the wild-type hepcidin mRNA. From sequencing analysis, this additional band was a selective splicing variant of hepcidin mRNA lacking exon 2 of HAMP gene, producing the transcript that encodes truncated peptide lacking 20 amino acids at the middle of preprohepcidin. In the present study, we used the digital PCR, because such a small amount of variant mRNA was difficult to quantitate by the conventional RT-PCR amplification. Among seven hepatoma-derived cell lines, six cell lines have significant copy numbers of this variant mRNA, but not in one cell line. In the transient transfection analysis of variant-type hepcidin cDNA, truncated preprohepcidin has a different character comparing with native preprohepcidin: its product is insensitive to digestion, and secreted into the medium as a whole preprohepcidin form without maturation. Loss or reduction of function of HAMP gene by aberrantly splicing may be a suitable phenomenon to obtain the proliferating advantage of hepatoma cells.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND\nlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2016.05.153.","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"27264950"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"622"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"666"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Toki, Yasumichi"},{"subitem_text_value":"Sasaki, Katsunori"},{"subitem_text_value":"Tanaka, Hiroki"},{"subitem_text_value":"Yamamoto, Masayo"},{"subitem_text_value":"Hatayama, Mayumi"},{"subitem_text_value":"Ito, Satoshi"},{"subitem_text_value":"Ikuta, Katsuya"},{"subitem_text_value":"Shindo, Motohiro"},{"subitem_text_value":"Hasebe, Takumu"},{"subitem_text_value":"Nakajima, Shunsuke"},{"subitem_text_value":"Sawada, Koji"},{"subitem_text_value":"Fujiya, Mikihiro"},{"subitem_text_value":"Torimoto, Yoshihiro"},{"subitem_text_value":"Ohtake, Takaaki"},{"subitem_text_value":"Kohgo, Yutaka"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"土岐, 康通"},{"creatorName":"トキ, ヤスミチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 勝則"},{"creatorName":"ササキ, カツノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 宏樹"},{"creatorName":"タナカ, ヒロキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山本, 昌代"},{"creatorName":"ヤマモト, マサヨ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"畑山, 真弓"},{"creatorName":"ハタヤマ, マユミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤, 巧"},{"creatorName":"イトウ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17831","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"生田, 克哉"},{"creatorName":"イクタ, カツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17832","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"進藤, 基博"},{"creatorName":"シンドウ, モトヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17833","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷部, 拓夢"},{"creatorName":"ハセベ, タクム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17834","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中嶋, 駿介"},{"creatorName":"ナカジマ, シュンスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17835","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"澤田, 康司"},{"creatorName":"サワダ, コウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17836","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤谷, 幹浩"},{"creatorName":"フジヤ, ミキヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鳥本, 悦宏"},{"creatorName":"トリモト, ヨシヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大竹, 孝明"},{"creatorName":"オオタケ, タカアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高後, 裕"},{"creatorName":"コウゴ, ユタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17840","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6771.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6771.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5706/files/6771.pdf"},"version_id":"3f8fbcc0-d8db-4ac8-b55d-7cc63a3ddb07"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aberrant splicing","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell lines","subitem_subject_scheme":"Other"},{"subitem_subject":"HAMP gene","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepatocellular carcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepcidin mRNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines"}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-01"},"publish_date":"2017-09-01","publish_status":"0","recid":"5706","relation_version_is_last":true,"title":["A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:14:47.843502+00:00"}