{"created":"2023-06-19T11:24:52.556621+00:00","id":5659,"links":{},"metadata":{"_buckets":{"deposit":"fbd8a90b-271a-46d8-b8bf-f1f994879d5d"},"_deposit":{"created_by":3,"id":"5659","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5659"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005659","sets":["7","7:27:41"]},"author_link":["17659"],"item_10_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"228","bibliographicPageStart":"217","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Journal of innate immunity"}]}]},"item_10_date_granted_30":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-24"}]},"item_10_degree_grantor_32":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"旭川医科大学"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID：28068663","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Collectins are C-type lectins that are involved in innate immunity as pattern recognition molecules. Recently, collectin kidney 1 (CL-K1) has been discovered, and in vitro studies have shown that CL-K1 binds to microbes and activates the lectin complement pathway. However, in vivo functions of CL-K1 against microbes have not been elucidated. To investigate the biological functions of CL-K1, we generated CL-K1 knockout (CL-K1-/-) mice and then performed a Streptococcus pneumoniae infection analysis. First, we found that recombinant human CL-K1 bound to S. pneumoniae in a calcium-dependent manner, and induced complement activation. CL-K1-/- mice sera formed less C3 deposition on S. pneumoniae. Furthermore, immunofluorescence analysis in the wild-type (WT) mice demonstrated that CL-K1 and C3 were localized on S. pneumoniae in infected lungs. CL-K1-/- mice revealed decreased phagocytosis of S. pneumoniae. Consequently, less S. pneumoniae clearance was observed in their lungs. CL-K1-/- mice showed severe pulmonary inflammation and weight loss in comparison with WT mice. Finally, the decreased clearance and severe pulmonary inflammation caused by S. pneumoniae infection might cause higher CL-K1-/- mice lethality. Our results suggest that CL-K1 might play an important role in host protection against S. pneumoniae infection through the activation of the lectin complement pathway.","subitem_description_type":"Abstract"}]},"item_10_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_dissertation_number_29":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"10107A507"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1159/000453316","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1662-811X","subitem_source_identifier_type":"ISSN"}]},"item_10_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Hwang, Insu"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"黄, 仁秀","creatorNameLang":"zh-cn"}],"nameIdentifiers":[{"nameIdentifier":"17659","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6709.pdf","filesize":[{"value":"499.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6709.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5659/files/6709.pdf"},"version_id":"853370a2-ccf4-432e-a888-e2d71090374c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Collectin kidney 1 (CL-K1) plays an important role in innate immunity against Streptococcus pneumoniae infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Collectin kidney 1 (CL-K1) plays an important role in innate immunity against Streptococcus pneumoniae infection","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["6","7","41"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-04-26"},"publish_date":"2017-04-26","publish_status":"0","recid":"5659","relation_version_is_last":true,"title":["Collectin kidney 1 (CL-K1) plays an important role in innate immunity against Streptococcus pneumoniae infection"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-09T06:58:21.536984+00:00"}