{"created":"2023-06-19T11:24:51.464291+00:00","id":5641,"links":{},"metadata":{"_buckets":{"deposit":"49f1a47c-01cf-400f-809a-21e593a32952"},"_deposit":{"created_by":1,"id":"5641","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5641"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005641","sets":["7","7:24"]},"author_link":["17616","17620","17619","17622","17618","17617","17621"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"Journal of Gastroenterology"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\n\\nNon-alcoholic fatty liver disease (NAFLD) is frequently accompanied by iron overload. However, because of the complex hepcidin-regulating molecules, the molecular mechanism underlying iron overload remains unknown. To identify the key molecule involved in NAFLD-associated iron dysregulation, we performed whole-RNA sequencing on the livers of obese mice.\nMETHODS:\n\\nMale C57BL/6 mice were fed a regular or high-fat diet for 16 or 48 weeks. Internal iron was evaluated by plasma iron, ferritin or hepatic iron content. Whole-RNA sequencing was performed by transcriptome analysis using semiconductor high-throughput sequencer. Mouse liver tissues or isolated hepatocytes and sinusoidal endothelial cells were used to assess the expression of iron-regulating molecules.\nRESULTS:\n\\nMice fed a high-fat diet for 16 weeks showed excess iron accumulation. Longer exposure to a high-fat diet increased hepatic fibrosis and intrahepatic iron accumulation. A pathway analysis of the sequencing data showed that several inflammatory pathways, including bone morphogenetic protein (BMP)-SMAD signaling, were significantly affected. Sequencing analysis showed 2314 altered genes, including decreased mRNA expression of the hepcidin-coding gene Hamp. Hepcidin protein expression and SMAD phosphorylation, which induces Hamp, were found to be reduced. The expression of BMP-binding endothelial regulator (BMPER), which inhibits BMP-SMAD signaling by binding BMP extracellularly, was up-regulated in fatty livers. In addition, immunohistochemical and cell isolation analyses showed that BMPER was primarily expressed in the liver sinusoidal endothelial cells (LSECs) rather than hepatocytes.\nCONCLUSIONS:\n\\nBMPER secretion by LSECs inhibits BMP-SMAD signaling in hepatocytes and further reduces hepcidin protein expression. These intrahepatic molecular interactions suggest a novel molecular basis of iron overload in NAFLD.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/） \n\\n2016 Jun 30.Epub","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00535-016-1237-6","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0944-1174","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"27364348"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"686"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"956"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Hasebe, Takumu"},{"subitem_text_value":"Tanaka, Hiroki"},{"subitem_text_value":"Sawada, Koji"},{"subitem_text_value":"Nakajima, Shunsuke"},{"subitem_text_value":"Ohtake, Takaaki"},{"subitem_text_value":"Fujiya, Mikihiro"},{"subitem_text_value":"Kohgo, Yutaka"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長谷部, 拓夢"},{"creatorName":"ハセベ, タクム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 宏樹"},{"creatorName":"タナカ, ヒロキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"澤田, 康司"},{"creatorName":"サワダ, コウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中嶋, 駿介"},{"creatorName":"ナカジマ, シュンスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大竹, 孝明"},{"creatorName":"オオタケ, タカアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17620","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤谷, 幹浩"},{"creatorName":"フジヤ, ミキヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高後, 裕"},{"creatorName":"コウゴ, ユタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17622","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6675.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6675.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5641/files/6675.pdf"},"version_id":"a923d841-2372-4caf-aff3-f4d5dfb12eb2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hepcidin","subitem_subject_scheme":"Other"},{"subitem_subject":"Iron metabolism","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-alcoholic fatty liver disease","subitem_subject_scheme":"Other"},{"subitem_subject":"Signal regulation","subitem_subject_scheme":"Other"},{"subitem_subject":"Whole-RNA sequencing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bone morphogenetic protein-binding endothelial regulator of liver sinusoidal endothelial cells induces iron overload in a fatty liver mouse model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bone morphogenetic protein-binding endothelial regulator of liver sinusoidal endothelial cells induces iron overload in a fatty liver mouse model"}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-16"},"publish_date":"2017-01-16","publish_status":"0","recid":"5641","relation_version_is_last":true,"title":["Bone morphogenetic protein-binding endothelial regulator of liver sinusoidal endothelial cells induces iron overload in a fatty liver mouse model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:16:39.181603+00:00"}