{"created":"2023-06-19T11:24:38.819179+00:00","id":5396,"links":{},"metadata":{"_buckets":{"deposit":"dd696213-a5fc-4531-9981-ca10b0fdd4ef"},"_deposit":{"created_by":1,"id":"5396","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5396"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005396","sets":["7","7:24"]},"author_link":["16938","16933","16937","16936","16934","16935"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"381","bibliographicPageStart":"370","bibliographicVolumeNumber":"87","bibliographic_titles":[{"bibliographic_title":"Kidney International"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/article/pii/S0085253815300570 | http://www.sciencedirect.com/science/article/pii/S0085253815300570","subitem_description_type":"Other"}]},"item_5_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID:25140913"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bolus administration of endothelin-1 elicits long-lasting renal afferent arteriolar vasoconstriction, in contrast to transient constriction induced by angiotensin II. Vasoconstriction is generally evoked by myosin regulatory light chain (LC20) phosphorylation at Ser19 by myosin light chain kinase (MLCK), which is enhanced by Rho-associated kinase (ROCK)-mediated inhibition of myosin light chain phosphatase (MLCP). LC20 can be diphosphorylated at Ser19 and Thr18, resulting in reduced rates of dephosphorylation and relaxation. Here we tested whether LC20 diphosphorylation contributes to sustained endothelin-1 but not transient angiotensin II-induced vasoconstriction. Endothelin-1 treatment of isolated arterioles elicited a concentration- and time-dependent increase in LC20 diphosphorylation at Thr18 and Ser19. Inhibition of MLCK or ROCK reduced endothelin-1-evoked LC20 mono- and diphosphorylation. Pretreatment with an ETB but not an ETA receptor antagonist abolished LC20 diphosphorylation, and an ETB receptor agonist induced LC20 diphosphorylation. In contrast, angiotensin II caused phosphorylation exclusively at Ser19. Thus, endothelin-1 and angiotensin II induce afferent arteriolar constriction via LC20 phosphorylation at Ser19 due to calcium activation of MLCK and ROCK-mediated inhibition of MLCP. Endothelin-1, but not angiotensin II, induces phosphorylation of LC20 at Thr18. This could contribute to the prolonged vasoconstrictor response to endothelin-1.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CC-BY-NC-ND","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/ki.2014.284"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0085-2538","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"25140913"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1048"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Takeya, Kosuke"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹谷, 浩介"},{"creatorName":"タケヤ, コウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Xuemei, Wang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iris, Kathol"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kathy, Loutzenhiser"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rodger, Loutzenhiser"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Michael, P. Walsh"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6385.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6385.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5396/files/6385.pdf"},"version_id":"9b0aecc4-86c5-45a0-aa70-dd7ff632f585"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"afferent arteriole","subitem_subject_scheme":"Other"},{"subitem_subject":"angiotensin II","subitem_subject_scheme":"Other"},{"subitem_subject":"diphosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"endothelin-1","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin regulatory light chain","subitem_subject_scheme":"Other"},{"subitem_subject":"Rho-associated kinase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Endothelin-1, but not angiotensin II, induces afferent arteriolar myosin diphosphorylation as a potential contributor to prolonged vasoconstriction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Endothelin-1, but not angiotensin II, induces afferent arteriolar myosin diphosphorylation as a potential contributor to prolonged vasoconstriction"}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-04-04"},"publish_date":"2016-04-04","publish_status":"0","recid":"5396","relation_version_is_last":true,"title":["Endothelin-1, but not angiotensin II, induces afferent arteriolar myosin diphosphorylation as a potential contributor to prolonged vasoconstriction"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:22:17.322154+00:00"}