| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2015-09-04 |
| タイトル |
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|
タイトル |
Low shear stress up-regulation of proinflammatory gene expression in human retinal microvascular endothelial cells. |
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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キーワード |
adhesion molecule |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
chemokine |
| キーワード |
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主題Scheme |
Other |
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キーワード |
cytokine |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
inflammation |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
procoagulant factor |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
retinal blood flow |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
retinal vascular endothelium |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
shear stress |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
石羽澤, 明弘
長岡, 泰司
横田, 陽匡
大野, 晋治
吉田, 晃敏
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| 著者 ローマ字 |
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Ishibazawa, Akihiro |
| 著者 ローマ字 |
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Nagaoka, Taiji |
| 著者 ローマ字 |
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Yokota, Harumasa |
| 著者 ローマ字 |
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Ono, Shinji |
| 著者 ローマ字 |
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Yoshida, Akitoshi |
| 書誌情報 |
Experimental Eye Research
巻 116,
p. 308-311,
発行日 2013-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0014-4835 |
| DOI |
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|
関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.exer.2013.10.001 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
|
内容記述 |
PMID:24128656 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The vascular endothelium responds to shear stress generated by blood flow and changes functions to regulate blood flow and maintain tissue homeostasis. Recently, we found that arteriolar high shear stress leads to increased expression of vasodilatory and antithrombotic genes in human retinal microvascular endothelial cells (HRMECs). However, it is unknown whether low shear stress, which is induced by hypoperfusion particularly in the retinal venules where leukocyte-endothelial interactions mainly occur, affects the retinal endothelial function. We studied the effect of low shear stress on proinflammatory gene expression in HRMECs. The cells were cultured on glass plates and exposed to laminar shear stresses of 0 (static), 1.5 (relatively low flow), and 15 dyne/cm(2) (relatively high flow) for 24 h using parallel plate-type flow-loading devices. The mRNA expressions of adhesion molecules, cytokines and chemokines, and procoagulant factors were evaluated using real-time reverse-transcription polymerase chain reaction. HRMECs exposed to 1.5 dyne/cm(2) significantly up-regulated the mRNA expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin. The cells exposed to 1.5 dyne/cm(2) of stress also had increased cytokine/chemokine mRNA expression, i.e., interleukin (IL)-6, IL-8, platelet-derived growth factor-B, and monocyte chemotactic protein-1. Procoagulant factors, i.e., tissue factor and plasminogen activator inhibitor-1 mRNA, increased significantly with exposure to 1.5 dyne/cm(2) of stress. Our results showed that relatively low shear stress causes up-regulation of proinflammatory genes in HRMECs, suggesting that decreased shear stress due to vascular hypoperfusion might change the phenotypic characterization of the retinal vascular endothelium and be associated with leukocyte-endothelial interactions. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
CC-BY-NC-ND |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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24128656 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
814 |
6192.pdf (203.6 kB)
