{"created":"2023-06-19T11:24:19.367514+00:00","id":5021,"links":{},"metadata":{"_buckets":{"deposit":"4de35c73-7fb4-4012-ba64-cc47fa7648ad"},"_deposit":{"created_by":1,"id":"5021","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5021"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005021","sets":["7","7:24"]},"author_link":["15700","15702","15699","15705","15701","15704","15703"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"218","bibliographicPageStart":"205","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Traffic"}]}]},"item_5_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID:23171199","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prior to secretion, regulated peptide hormones are selectively sorted to secretory granules (SGs) at the trans-Golgi network (TGN) in endocrine cells. Secretogranin III (SgIII) appears to facilitate SG sorting process by tethering of protein aggregates containing chromogranin A (CgA) and peptide hormones to the cholesterol-rich SG membrane (SGM). Here, we evaluated the role of SgIII in SG sorting in AtT-20 cells transfected with small interfering RNA targeting SgIII. In the SgIII-knockdown cells, the intracellular retention of CgA was greatly impaired, and only a trace amount of CgA was localized within the vacuoles formed in the TGN, confirming the significance of SgIII in both the tethering of CgA-containing aggregates and the establishment of the proper SG morphology. Although the intracellular retention of proopiomelanocortin (POMC) was considerably impaired in SgIII-knockdown cells, residual adrenocorticotropic hormone (ACTH)/POMC was still localized to some few remaining SGs together with another granin protein, secretogranin II (SgII), and was secreted in a regulated manner. Biochemical analyses indicated that SgII bound directly to the SGM in a cholesterol-dependent manner and was able to retain the aggregated form of POMC, revealing a latent redundancy in the SG sorting and retention mechanisms, that ensures the regulated secretion of bioactive peptides.\n\\n© 2012 John Wiley & Sons A/S.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"author","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/tra.12029","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1398-9219","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"23171199"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"726"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1245"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Watanabe, Tsuyoshi"},{"subitem_text_value":"Bochimoto, Hiroki"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Torii, Seiji"},{"subitem_text_value":"Takeuchi, Toshiyuki"},{"subitem_text_value":"Hosaka, Masahiro"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sun, Meng"}],"nameIdentifiers":[{"nameIdentifier":"15699","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"渡部, 剛"},{"creatorName":"ワタナベ, ツヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"15700","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"暮地本, 宙己"},{"creatorName":"ボチモト, ヒロキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"15701","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakai, Yuko"}],"nameIdentifiers":[{"nameIdentifier":"15702","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鳥居, 征司"},{"creatorName":"トリイ, セイジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"15703","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"竹内, 利行"},{"creatorName":"タケウチ, トシユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"15704","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"穂坂, 正博"},{"creatorName":"ホサカ, マサヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"15705","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"5890.pdf","filesize":[{"value":"6.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"5890.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5021/files/5890.pdf"},"version_id":"4ba1ee2e-a06e-4d7e-9adf-68c0dafb63a2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"secretogranin III","subitem_subject_scheme":"Other"},{"subitem_subject":"secretogranin II","subitem_subject_scheme":"Other"},{"subitem_subject":"chromogranin A","subitem_subject_scheme":"Other"},{"subitem_subject":"cholesterol","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide hormones","subitem_subject_scheme":"Other"},{"subitem_subject":"prohormone sorting","subitem_subject_scheme":"Other"},{"subitem_subject":"secretory granule","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Multiple sorting systems for secretory granules ensure the regulated secretion of peptide hormones.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Multiple sorting systems for secretory granules ensure the regulated secretion of peptide hormones."}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-30"},"publish_date":"2014-10-30","publish_status":"0","recid":"5021","relation_version_is_last":true,"title":["Multiple sorting systems for secretory granules ensure the regulated secretion of peptide hormones."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:31:30.152113+00:00"}