{"created":"2023-06-19T11:23:41.907792+00:00","id":4275,"links":{},"metadata":{"_buckets":{"deposit":"9860236f-bad1-4d52-aeaf-b94a9c1737eb"},"_deposit":{"created_by":3,"id":"4275","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4275"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004275","sets":["7","7:24"]},"author_link":["13245","13246","13247","13248","13249","13250","13251","13252","13253","13254","13255","13256"],"control_number":"4275","item_1716186501932":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"23097189","subitem_relation_type_select":"PMID"}}]},"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"843","bibliographicPageStart":"831","bibliographicVolumeNumber":"114","bibliographic_titles":[{"bibliographic_title":"Journal of cellular biochemistry"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously showed that mature hepatocytes could transdifferentiate into bile ductular cells when placed in a collagen-rich microenvironment. To explore the mechanism of transdifferentiation, we examined whether inflammatory cytokines affected the phenotype of hepatocytes in a three-dimensional culture system. Spheroidal aggregates of rat hepatocytes were embedded within a type I collagen gel matrix and cultured in the presence of various cytokines. In the control, hepatocytes gradually lost expression of albumin, tyrosine aminotransferase, and hepatocyte nuclear factor (HNF)-4α, while aberrantly expressed bile ductular markers, including cytokeratin 19 (CK 19) and spermatogenic immunoglobulin superfamily (SgIGSF). Among the cytokines examined, tumor necrosis factor (TNF)-α inhibited expression of albumin and HNF-4α, both at mRNA and protein levels. After culturing for 2 weeks with TNF-α, hepatocytic spheroids were transformed into extensively branching tubular structures composed of CK 19- and SgIGSF-positive small cuboidal cells. These cells responded to secretin with an increase in secretion and expressed functional bile duct markers. TNF-α also induced the phosphorylation of Jun N-terminal kinase (JNK) and c-Jun, and the morphogenesis was inhibited by SP600125, a specific JNK inhibitor. Furthermore, in chronic rat liver injury induced by CCl(4) , ductular reaction in the centrilobular area demonstrated strong nuclear staining of phosphorylated c-Jun. Our results demonstrate that TNF-α promotes the ductular transdifferentiation of hepatocytes and suggest a role of TNF-α in the pathogenesis of ductular reaction.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"Author(preprint)","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/jcb.24424","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0730-2312","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"23097189"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"922"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"958"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西川, 祐司","creatorNameLang":"ja"},{"creatorName":"ニシカワ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"13245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sone, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagahama, Yasuharu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kumagai, Eriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Doi, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Omori, Yasufumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshioka, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokairin, Takuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13253","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Yohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13254","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13255","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugiyama, Toshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13256","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Enomoto, Katsuhiko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"5017.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"5017.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/4275/files/5017.pdf"},"version_id":"b1c59e6d-df61-4d1a-8096-a8a637193c35"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ductular reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transdifferentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tumor necrosis factor-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Liver fibrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepatocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bile duct cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"JNK-c-Jun pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tumor necrosis factor-α promotes bile ductular transdifferentiation of mature rat hepatocytes in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor necrosis factor-α promotes bile ductular transdifferentiation of mature rat hepatocytes in vitro","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-04"},"publish_date":"2013-02-04","publish_status":"0","recid":"4275","relation_version_is_last":true,"title":["Tumor necrosis factor-α promotes bile ductular transdifferentiation of mature rat hepatocytes in vitro"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-08-09T07:30:21.509103+00:00"}