{"created":"2023-06-19T11:23:31.465327+00:00","id":4083,"links":{},"metadata":{"_buckets":{"deposit":"b77968dc-9bdb-411f-90db-d5ab4fa7d955"},"_deposit":{"created_by":3,"id":"4083","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4083"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004083","sets":["7","7:24"]},"author_link":["12478"],"control_number":"4083","item_5_alternative_title_3":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"凍結乾燥した精子における染色体完全性とDNA損傷","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"210","bibliographicPageStart":"199","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Reproductive Medicine and Biology"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Freeze-drying technology may one day be used to preserve mammalian spermatozoa indefinitely without cryopreservation. Freeze-dried mouse spermatozoa stored below 4°C for up to 1 year have maintained the ability to fertilize oocytes and support normal development. The maximum storage period for spermatozoa increases at lower storage temperatures. Freeze-drying, per se, may reduce the integrity of chromosomes in freeze-dried mouse spermatozoa, but induction of chromosomal damage is suppressed if spermatozoa are incubated with divalent cation chelating agents prior to freeze-drying. Nevertheless, chromosomal damage does accumulate in spermatozoa stored at temperatures above 4°C. Currently, no established methods or strategies can prevent or reduce damage accumulation, and damage accumulation during storage is a serious obstacle to advances in freeze-drying technology. Chromosomal integrity of freeze-dried human spermatozoa have roughly background levels of chromosomal damage after storage at 4°C for 1 month, but whether these spermatozoa can produce healthy newborns is unknown. The safety of using freeze-dried human spermatozoa must be evaluated based on the risks of heritable chromosome and DNA damage that accumulates during storage.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"Author","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1445-5781","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"LC21260001"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"819"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"870"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日下部, 博一","creatorNameLang":"ja"},{"creatorName":"クサカベ, ヒロカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"12478","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"4799.pdf","filesize":[{"value":"274.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"4799.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/4083/files/4799.pdf"},"version_id":"21dd7ee5-9fe7-4816-924e-de9d3590ec01"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chromosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryopreservation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA damage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Freeze-drying","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spermatozoa","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chromosomal integrity and DNA damage in freeze-dried spermatozoa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chromosomal integrity and DNA damage in freeze-dried spermatozoa","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-09-12"},"publish_date":"2012-09-12","publish_status":"0","recid":"4083","relation_version_is_last":true,"title":["Chromosomal integrity and DNA damage in freeze-dried spermatozoa"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-07-30T00:36:27.282354+00:00"}