{"created":"2023-06-19T11:23:30.300133+00:00","id":4058,"links":{},"metadata":{"_buckets":{"deposit":"fcfe787b-8470-4f11-bcc4-e1d354d4a55e"},"_deposit":{"created_by":3,"id":"4058","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4058"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004058","sets":["7","7:23"]},"author_link":["12384","12385","12386","12387","12388","12389"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"452","bibliographicPageStart":"447","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"脈管学"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"雑種成犬胸管よりリンパ管内皮細胞(LEC)を単離して培養細胞系を確立した。これらの細胞はLEC特異マーカーのVEGFR-3、LYVE-1、Podoplanin、Prox1の全てに陽性であり、肝細胞増殖因子(HGF)の受容体c-Metの発現を認めた。LECにヒトrecombinant HGFを添加した場合、細胞増殖能と遊走能は濃度依存性に増加した。ヒトHGFプラスミドDNAと、c-fosプロモーターの転写因子結合部位下流にLuciferase遺伝子を挿入したレポーター遺伝子をLECに共導入した場合、有意に増殖能の増加を認めた。HGFがタンパク、遺伝子のいずれにおいてもLECの増殖・遊走作用を有することが確認できた。LECをヒトrecombinant HGFで刺激した場合、ERK、Aktともリン酸化を認めた。HGF刺激前にMEK inhibitorまたはPI3K inhibitorで処理した場合、LECの増殖能、遊走能が阻害された。ラットのリンパ浮腫モデルにHGF遺伝子を導入し、浮腫の有意な改善を認めた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"雑誌掲載版","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0387-1126","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2012118524"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"846"}]},"item_5_text_7":{"attribute_name":"著者 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