{"created":"2023-06-19T11:23:29.753591+00:00","id":4046,"links":{},"metadata":{"_buckets":{"deposit":"fe2d09dc-6219-41cd-a6b5-0bfe73fa48f3"},"_deposit":{"created_by":3,"id":"4046","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4046"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004046","sets":["7","7:24"]},"author_link":["12320","12321","12322","12323"],"control_number":"4046","item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"32","bibliographicPageEnd":"24547","bibliographicPageStart":"24538","bibliographicVolumeNumber":"285","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have developed a stable analog for the ADP-insensitive phosphoenzyme intermediate with two occluded Ca^<2+> at the transport sites (E2PCa_2) of sarcoplasmic reticulum Ca^<2+>-ATPase. This is normally a transient intermediate state during phosphoenzyme isomerization from the ADP-sensitive to ADP-insensitive form and Ca^<2+> deocclusion/release to the lumen; E1PCa_2 → E2PCa_2 → E2P + 2Ca^<2+>. Stabilization was achieved by elongation of the Glu^<40>-Ser^<48> loop linking the Actuator domain and M1 (1st transmembrane helix) with four glycine insertions at Gly^<46>/Lys^<47> and by binding of beryllium fluoride (BeFx) to the phosphorylation site of the Ca^<2+>-bound ATPase (E1Ca_2). The complex E2Ca_2·BeF_3− was also produced by lumenal Ca^<2+> binding to E2·BeF_3− (E2P ground state analog) of the elongated linker mutant. The complex was stable for at least 1 week at 25℃. Only BeFx, but not AlFx or MgFx, produced the E2PCa_2 structural analog. Complex formation required binding of Mg^<2+>, Mn^<2+>, or Ca^<2+> at the catalytic Mg^<2+> site. Results reveal that the phosphorylation product E1PCa_2 and the E2P ground state (but not the transition states) become competent to produce the E2PCa_2 transient state during forward and reverse phosphoenzyme isomerization. Thus, isomerization and lumenal Ca^<2+> release processes are strictly coupled with the formation of the acylphosphate covalent bond at the catalytic site. Results also demonstrate the critical structural roles of the Glu^<40>-Ser^<48> linker and of Mg^<2+> at the catalytic site in these processes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M110.144535","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"20529842"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1025"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"822"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大保, 貴嗣","creatorNameLang":"ja"},{"creatorName":"ダイホ, タカシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"12320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Danko, Stefania","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamasaki, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12322","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12323","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"4540.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"4540.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/4046/files/4540.pdf"},"version_id":"487458f9-f37f-47c6-a227-24c71365fa1a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stable Structural Analog of Ca^<2+>-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca^<2+> Formed by Elongation of A-domain/M1′-linker and Beryllium Fluoride Binding","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stable Structural Analog of Ca^<2+>-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca^<2+> Formed by Elongation of A-domain/M1′-linker and Beryllium Fluoride Binding","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-05-17"},"publish_date":"2012-05-17","publish_status":"0","recid":"4046","relation_version_is_last":true,"title":["Stable Structural Analog of Ca^<2+>-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca^<2+> Formed by Elongation of A-domain/M1′-linker and Beryllium Fluoride Binding"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-07-30T00:59:07.355746+00:00"}