{"created":"2023-06-19T11:20:17.328362+00:00","id":311,"links":{},"metadata":{"_buckets":{"deposit":"2e95c7f3-813d-42ac-a85e-9d62a7efc026"},"_deposit":{"created_by":3,"id":"311","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"311"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000311","sets":["7","7:24"]},"author_link":["590","591","592","593","594","595","596","597","598"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"981","bibliographicPageStart":"976","bibliographicVolumeNumber":"280","bibliographic_titles":[{"bibliographic_title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/journal/0006291X | http://www.sciencedirect.com/science/journal/0006291X","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Orexin-A is a neuropeptide consisting 33 amino acids with two intrachain disulfide bonds, namely Cys6-Cys12 and Cys7-Cys14, and is a potent stimulator of food consumption and gastric acid secretion. In contrast, orexin-B, a peptide containing 28 amino acids without disulfide bond, which has no stimulatory action of gastric acid. The objective of the present study was to characterize the receptor-mediated mechanism of orexin-A-induced stimulation of gastric acid secretion using orexin-Arelated peptides with modification of disulfide bonds. Intracisternal injection of orexin-A but not orexin-B or orexin-A (15-33) that does not contain both disulfide bonds stimulated gastric acid secretion in pylorusligated conscious rats. The ability of the stimulation of gastric acid output was less in three alanine-substituted orexin-A, [Ala^(6, 12)]orexin-A, [Ala^(7, 14)]orexin-A and [Ala^(6, 7, 12, 14)]orexin-A, than orexin-A. Orexins-induced calcium increase was measured in CHO-K1 cells expressing OX_1R or OX_2R. Orexin-A induced a transient increase in [Ca^(2+)]I in CHO-K1/OX_1R cells in a dose-dependent manner. EC_50 values for OX_1R of orexin-A, orexin-B or orexin-A (15-33) was 0.068, 0.69 or 4.1 nM, respectively, suggesting that peptides containing no disulfide bonds have lower potency for the receptor. Agonistic activity for OX1R of the three orexin-A analogues with modification of one or both disulfide bonds was significantly reduced as compared with that of orexin-A. EC_50 values for OX_2R of orexin-A and orexin-B was almost equal but potency for the receptor of orexin-A (15-33) and three alanine substituted orexin-A was less than that of orexin-A. A significant inverse relationship between gastric acid output and EC_50 values for OX_1R but not OX_2R was observed. These results suggested that the  orexin-A-induced acid stimulation requires OX_1R activation and that disulfide bonds in orexin-A may have a key role in the receptor activation.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"author","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1006/bbrc.2001.4235","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"11162621"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2302"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"943"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Okumura, Toshikatsu"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"奥村, 利勝"},{"creatorName":"オクムラ, トシカツ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"590","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeuchi, S"}],"nameIdentifiers":[{"nameIdentifier":"591","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motomura, W"}],"nameIdentifiers":[{"nameIdentifier":"592","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, H"}],"nameIdentifiers":[{"nameIdentifier":"593","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Egashira, S"}],"nameIdentifiers":[{"nameIdentifier":"594","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asahi, S"}],"nameIdentifiers":[{"nameIdentifier":"595","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanatani, A"}],"nameIdentifiers":[{"nameIdentifier":"596","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ihara, M"}],"nameIdentifiers":[{"nameIdentifier":"597","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kohgo, Y"}],"nameIdentifiers":[{"nameIdentifier":"598","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"384.pdf","filesize":[{"value":"230.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"384.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/311/files/384.pdf"},"version_id":"7c518018-24c8-45e1-9f82-9f20769e6369"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Requirement of intact disulfide bonds in orexin-A-induced stimulation of gastric acid secretion that is mediated by OX1 receptor activation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Requirement of intact disulfide bonds in orexin-A-induced stimulation of gastric acid secretion that is mediated by OX1 receptor activation","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-03-22"},"publish_date":"2007-03-22","publish_status":"0","recid":"311","relation_version_is_last":true,"title":["Requirement of intact disulfide bonds in orexin-A-induced stimulation of gastric acid secretion that is mediated by OX1 receptor activation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:20:09.916698+00:00"}