{"created":"2023-06-19T11:20:16.399856+00:00","id":294,"links":{},"metadata":{"_buckets":{"deposit":"9f24e6d9-1de0-493e-9915-8cd94d8d8736"},"_deposit":{"created_by":3,"id":"294","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"294"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000294","sets":["7","7:24"]},"author_link":["521","522","523","524","525"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"531","bibliographicPageStart":"527","bibliographicVolumeNumber":"267","bibliographic_titles":[{"bibliographic_title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/journal/0006291X | http://www.sciencedirect.com/science/journal/0006291X","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It has been shown that intracerebroventricular injection of synthetic orexins stimulated food intake in rats. This pharmacological evidence suggests that orexins may have a role for the central regulation of feeding. In the present study, we investigated the hypothesis whether endogenous orexin indeed play a vital role in feeding behavior. An anti-orexin polyclonal antibody was used throughout the study. First, we examined the specificity of the antibody to orexin by Western blot analysis and immunohistochemistry. Next, the effects of central injection of the orexin antibody on food intake in 24-hr-fasted rats were evaluated. Western blot analysis revealed that the orexin antibody detected synthetic orexin-A.Immunohistochemical study showed that orexin-positive neurons were identified only in the lateral hypothalamic area, being in agreement with previous reports. Neither control antibody nor the orexin-antibody preabsorbed with excess amount of orexin-A detected neurons, indicating the orexin antibody is specific. Intracisternal but not intraperitoneal injection of the orexin antibody dose-dependently suppressed feeding. All these results suggest that immunoneutralization of endogenous orexins in the brain reduced food intake. In other words, we suggest that endogenous brain orexin may have a physiologically relevant action on feeding behavior.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"ELSEVIER, Yamada, H; Okumura, T; Motomura, W; Kobayashi, Y; Kohgo, Y, BBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 267(2), 527-531, 2000.\n\\nauthor","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1006/bbrc.1999.1998","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"10631095"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2768"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"998"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Okumura, Toshikatsu"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"奥村, 利勝"},{"creatorName":"オクムラ, トシカツ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, H"}],"nameIdentifiers":[{"nameIdentifier":"522","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motomura, W"}],"nameIdentifiers":[{"nameIdentifier":"523","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Y"}],"nameIdentifiers":[{"nameIdentifier":"524","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kohgo, Y"}],"nameIdentifiers":[{"nameIdentifier":"525","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"359.pdf","filesize":[{"value":"241.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"359.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/294/files/359.pdf"},"version_id":"0e76a2ba-d333-48c5-ae01-3d1a3b0d01a0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibition of food intake by central injection of anti-orexin antibody in fasted rats","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibition of food intake by central injection of anti-orexin antibody in fasted rats","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-03-22"},"publish_date":"2007-03-22","publish_status":"0","recid":"294","relation_version_is_last":true,"title":["Inhibition of food intake by central injection of anti-orexin antibody in fasted rats"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:20:23.741508+00:00"}