| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-03-22 |
| タイトル |
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タイトル |
Inhibition of food intake by central injection of anti-orexin antibody in fasted rats |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
奥村, 利勝
Yamada, H
Motomura, W
Kobayashi, Y
Kohgo, Y
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| 著者 ローマ字 |
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|
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Okumura, Toshikatsu |
| 書誌情報 |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
巻 267,
号 2,
p. 527-531,
発行日 2000-01-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1006/bbrc.1999.1998 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.sciencedirect.com/science/journal/0006291X | http://www.sciencedirect.com/science/journal/0006291X |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
It has been shown that intracerebroventricular injection of synthetic orexins stimulated food intake in rats. This pharmacological evidence suggests that orexins may have a role for the central regulation of feeding. In the present study, we investigated the hypothesis whether endogenous orexin indeed play a vital role in feeding behavior. An anti-orexin polyclonal antibody was used throughout the study. First, we examined the specificity of the antibody to orexin by Western blot analysis and immunohistochemistry. Next, the effects of central injection of the orexin antibody on food intake in 24-hr-fasted rats were evaluated. Western blot analysis revealed that the orexin antibody detected synthetic orexin-A.Immunohistochemical study showed that orexin-positive neurons were identified only in the lateral hypothalamic area, being in agreement with previous reports. Neither control antibody nor the orexin-antibody preabsorbed with excess amount of orexin-A detected neurons, indicating the orexin antibody is specific. Intracisternal but not intraperitoneal injection of the orexin antibody dose-dependently suppressed feeding. All these results suggest that immunoneutralization of endogenous orexins in the brain reduced food intake. In other words, we suggest that endogenous brain orexin may have a physiologically relevant action on feeding behavior. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
ELSEVIER, Yamada, H; Okumura, T; Motomura, W; Kobayashi, Y; Kohgo, Y, BBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 267(2), 527-531, 2000.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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10631095 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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998 |
359.pdf (241.9 kB)
