| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2010-08-02 |
| タイトル |
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タイトル |
Nigral GABAergic inhibition upon cholinergic neurons in the rat pedunculopontine tegmental nucleus |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
高草木, 薫
Saito, K
Hattori, S
Song, WJ
Isa, T
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| 著者 ローマ字 |
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Takakusaki, Kaoru |
| 著者 ローマ字 |
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en |
| 書誌情報 |
European Journal of Neuroscience
巻 18,
号 4,
p. 879-886,
発行日 2003-08-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0953-816X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1046/j.1460-9568.2003.02825.x |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://dx.doi.org/10.1046/j.1460-9568.2003.02825.x | http://dx.doi.org/10.1046/j.1460-9568.2003.02825.x |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We investigated, in a midbrain parasagittal slice preparation of Wistar rats (postnatal day 9-17), the synaptic inhibition of neurons in the pedunculopontine tegmental nucleus (PPN), which was mediated by gamma (gamma)-amino-butyric acid (GABA). Whole-cell patch-clamp recording was used, in combination with a single-cell reverse transcription-polymerase chain reaction amplification technique, to record synaptic potentials and to identify the phenotype of the recorded PPN neuron. In the presence of the ionotropic glutamate receptor antagonists, 6-cyano-2, 3-dihydroxy-7-nitro-quinoxaline-2, 3, dione, and dl-2-amino-5-phosphonovaleric acid, single electrical stimuli were applied to the substantia nigra pars reticulata (SNr), one of the basal ganglia output nuclei. Stimulation of the SNr evoked inhibitory postsynaptic potentials (IPSPs) in 73 of the 104 neurons in the PPN. The IPSPs were abolished with a GABAA receptor antagonist, bicuculline. Inhibitory postsynaptic currents of the neurons were reversed in polarity at approximately -93.5 mV, which was close to the value of the equilibrium potential for chloride ions of -88.4 mV. Single-cell reverse transcription-polymerase chain reactions revealed that approximately 30% (9/32) of the PPN neurons that received inhibition from the SNr expressed detectable levels of choline acetyltransferase mRNA. These findings show that output from the SNr regulates the activity of cholinergic PPN neurons through GABAA receptors. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
The definitive version is available at www.3.interscience.wiley.com |
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言語 |
en |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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12925013 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1325 |
3131.pdf (1.5 MB)
