| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2010-03-17 |
| タイトル |
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タイトル |
Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
伊藤, 亮
Nkouawa, A
Sako, Y
Nakao, M
Nakaya, K
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| 著者 ローマ字 |
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Ito, A |
| 著者 ローマ字 |
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en |
| 書誌情報 |
Journal of Clinical Microbiology
巻 47,
号 1,
p. 168-174,
発行日 2009-01-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0095-1137 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JCM.01573-08 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Loop-Mediated%20Isothermal%20Amplification%20Method%20for%20Differentiation%20and%20Rapid%20Detection%20of%20Taenia%20Species | http://www.ncbi.nlm.nih.gov/pubmed?term=Loop-Mediated%20Isothermal%20Amplification%20Method%20for%20Differentiation%20and%20Rapid%20Detection%20of%20Taenia%20Species |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Rapid detection and differentiation of Taenia species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are endemic. Because of the lower sensitivity and specificity of the conventional diagnosis based on microscopical examination, molecular tools are more reliable for differential diagnosis of these diseases. In this study, we developed and evaluated a loop-mediated isothermal amplification (LAMP) assay for differential diagnosis of infections with Taenia species with cathepsin L-like cysteine peptidase (clp) and cytochrome c oxidase subunit 1 (cox1) genes. LAMP with primer sets to the cox1 gene could differentiate between three species, and LAMP with primer sets to the clp gene could differentiate Taenia solium from Taenia saginata/Taenia asiatica. Restriction enzyme digestion of the LAMP products from primer set Tsag-clp allowed the differentiation of Taenia saginata from Taenia asiatica. We demonstrated the high specificity of LAMP by testing known parasite DNA samples extracted from proglottids (n = 100) and cysticerci (n = 68). LAMP could detect one copy of the target gene or five eggs of T. asiatica and T. saginata per gram of feces, showing sensitivity similar to that of PCR methods. Furthermore, LAMP could detect parasite DNA in all taeniid egg-positive fecal samples (n = 6). Due to the rapid, simple, specific, and sensitive detection of Taenia species, the LAMP assays are valuable tools which might be easily applicable for the control and prevention of taeniasis and cysticercosis in countries where these diseases are endemic. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright American Society for Microbiology, Journal of Clinical Microbiology, volume 47, 168-174, 2009 |
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言語 |
en |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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19005142 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1415 |
2886.pdf (868.2 kB)
