| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2025-05-28 |
| タイトル |
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タイトル |
miR-514a promotes neuronal development in human iPSC-derived neurons |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
IPSC |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
dendrite |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
mTOR |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
miR-514a |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
microRNA |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
neuronal development |
| 資源タイプ |
|
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資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Yuichi, Akaba
Satoru, Takahashi
Keiichiro, Suzuki
Kenjiro, Kosaki
Keita, Tsujimura
|
| bibliographic_information |
en : Frontiers in cell and developmental biology
巻 11,
p. 1096463,
発行日 2023-02-07
|
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2296-634X |
| DOI |
|
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3389/fcell.2023.1096463 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1096463/full |
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言語 |
en |
| item_1716186501932 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
36824367 |
| item_5_description_33 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Proper development and function of the central nervous system require precise regulation of gene expression. MicroRNAs (miRNAs), a group of small non-coding RNAs that can negatively regulate gene expression at the post-transcriptional level, are critical regulators of neuronal development, and dysregulation of microRNAs has been implicated in various neurological disorders. Changes in microRNA expression and repertoire are related to the emergence of social and behavioral variations in closely related primates, including humans, during evolution. MicroRNA-514a (miR-514a) is an X-linked miRNA that is conserved in species with higher social and cognitive functions, and frequent tandem duplications of miR-514a have been found in primate genomes. Here, we demonstrate that miR-514a plays a crucial role in neuronal development in neurons derived from human induced pluripotent stem cells (iPSCs). Overexpression of miR-514a increased dendritic length, soma size, and activity levels of mammalian target of rapamycin (mTOR) signaling in induced pluripotent stem cell-derived neurons, whereas blocking of endogenous miR-514a inhibited neuronal development. Furthermore, we performed a functional analysis of the miR-514a variation found during primate evolution, to investigate the impact of miR-514a sequence variation and associated changes in expression on brain development during evolution. We found that mutation in miR-514a significantly reduced the expression of the mature form and abolished the effects observed when native miR-514a was expressed. Our findings provide new insights into the functional role of miR-514a in the regulation of neuronal development and evolution of primate brain development. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
|
言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
| item_5_textarea_42 |
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en |
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© 2023 Akaba, Takahashi, Suzuki, Kosaki and Tsujimura |
36824367.pdf (2.5 MB)
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