| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2025-05-21 |
| タイトル |
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タイトル |
Electrostatic interactions between single arginine and phospholipids modulate physiological properties of sarcoplasmic reticulum Ca2+-ATPase |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Kazuo, Yamasaki
Takashi, Daiho
Satoshi, Yasuda
Stefania, Danko
Jun-Ichi, Kawabe
Hiroshi, Suzuki
|
| bibliographic_information |
en : Scientific Reports
巻 12,
号 1,
p. 12200,
発行日 2022-07-16
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| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2045-2322 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-022-16091-9 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
https://www.nature.com/articles/s41598-022-16091-9 |
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言語 |
en |
| item_1716186501932 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
35842495 |
| item_5_description_33 |
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内容記述タイプ |
Abstract |
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内容記述 |
Arg324 of sarcoplasmic reticulum Ca2+-ATPase forms electrostatic interactions with the phosphate moiety of phospholipids in most reaction states, and a hydrogen bond with Tyr122 in other states. Using site-directed mutagenesis, we explored the functional roles of Arg324 interactions, especially those with lipids, which at first glance might seem too weak to modulate the function of such a large membrane protein. The hydrogen bond forms transiently and facilitates Ca2+ binding from the cytoplasmic side. The contributions of the electrostatic interactions to the reaction steps were quantified using a rate vs activity coefficient plot. We found that the interaction between Arg324 and lipids decreases the affinity for luminal Ca2+. The transformation rate of the phosphoenzyme intermediate is facilitated by the electrostatic interactions, and the function of these interactions depends not only on the type but also on the composition of the phospholipids. The properties observed in microsomes could not be reproduced with any single phospholipid, but with a mixture of phospholipids that mimics the native membrane. These results suggest the importance of swapping of the lipid partners of different headgroups in the reaction step. This study shows that Arg324 plays a role in the reaction cycle via complex intra-protein and protein-lipid interactions. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
| item_5_textarea_42 |
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en |
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© 2022. The Author(s). |
35842495.pdf (1.6 MB)
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