{"created":"2025-01-20T04:15:39.189507+00:00","id":2000274,"links":{},"metadata":{"_buckets":{"deposit":"55f5fd30-9606-42fd-8a52-446a86815799"},"_deposit":{"created_by":3,"id":"2000274","owner":"3","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2000274"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:02000274","sets":["7:27:45"]},"author_link":[],"item_10_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"トリプルネガティブ乳癌における，血中循環腫瘍DNAの遺伝子解析と再発マーカ―としての可能性に関する検討","subitem_alternative_title_language":"ja"}]},"item_10_biblio_info_21":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"420","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"Oncology lettersletters","bibliographic_titleLang":"en"}]}]},"item_10_date_granted_30":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-03-25"}]},"item_10_degree_grantor_32":{"attribute_name":"item_10_degree_grantor_32","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"旭川医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10107","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID:33841581","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"item_10_description_33","attribute_value_mlt":[{"subitem_description":"Triple-negative breast cancer (TNBC) has a poorer prognosis than other breast cancer subtypes; therefore, identifying markers of early recurrence is important. The present study aimed to establish a liquid biopsy protocol for droplet digital PCR-based detection of frequently mutated genes in patients with TNBC. Tumor DNA from 36 patients with TNBC who relapsed within 2 years after surgical resection was retrospectively analyzed. Somatic mutational profiles were evaluated using targeted sequencing to identify frequently mutated genes and genes associated with molecularly targeted therapies. The association between genetic alterations and associated protein phosphorylation was investigated using immunohistochemical analysis. Recurrent hot spot mutations in the plasma were monitored over time. Mutation-specific probes were used to successfully detect mutations in the blood samples of patients who were positive for PIK3CA H1047R and AKT1 E17K mutations. Somatic mutations in AKT1 (14.9%) and PIK3CA (25.5%) were frequently identified in the data. Robust phosphorylation of AKT and S6RP was more common in tumors with PIK3CA H1047R and AKT1 E17K mutational background than in tumors with wild-type PIK3CA and AKT1. In conclusion, the present study evaluated a high-sensitivity detection system for frequently mutated genes that was also applicable for cell-free DNA. The PI3K/AKT pathway was revealed to be activated in patients harboring PIK3CA H1047R and AKT1 E17K mutations; therefore, the PI3K/AKT pathway may be a promising candidate for targeted therapy in these patients.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_dissertation_number_29":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第556号"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/ol.2021.12681","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1792-1074","subitem_source_identifier_type":"PISSN"}]},"item_10_version_type_38":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡崎, 智","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"オカザキ, サトシ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"},{"creatorName":"Okazaki, Satoshi","creatorNameLang":"en","creatorNameType":"Personal"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-11-04"}],"filename":"K556 Okazaki Satoshi_TD.pdf","filesize":[{"value":"12.5 MB"}],"format":"application/pdf","url":{"objectType":"fulltext","url":"https://asahikawa-med.repo.nii.ac.jp/record/2000274/files/K556 Okazaki Satoshi_TD.pdf"},"version_id":"a33b9f2a-1c46-4dd0-9725-df600937fe37"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"digital PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"early recurrence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic testing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid biopsy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"triple-negative breast cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"The feasibility of circulating tumor DNA analysis as a marker of recurrence in triple-negative breast cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The feasibility of circulating tumor DNA analysis as a marker of recurrence in triple-negative breast cancer","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["45"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-04"},"publish_date":"2021-11-04","publish_status":"0","recid":"2000274","relation_version_is_last":true,"title":["The feasibility of circulating tumor DNA analysis as a marker of recurrence in triple-negative breast cancer"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2025-01-20T04:21:52.579060+00:00"}