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Ability to activate oocytes and chromosome integrity of mouse spermatozoa preserved in EGTA Tris-HCl buffered solution supplemented with antioxidants
https://asahikawa-med.repo.nii.ac.jp/records/147
https://asahikawa-med.repo.nii.ac.jp/records/147d4dca451-3118-4d2a-802f-90fbcb027c76
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
227.pdf (283.4 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||
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| 公開日 | 2007-02-14 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Ability to activate oocytes and chromosome integrity of mouse spermatozoa preserved in EGTA Tris-HCl buffered solution supplemented with antioxidants | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
日下部, 博一
× 日下部, 博一
× Kamiguchi, Y
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| 著者 ローマ字 | ||||||||||||
| Kusakabe, Hirokazu | ||||||||||||
| 出版社 ローマ字 | ||||||||||||
| ELSEVIER SCIENCE INC | ||||||||||||
| 書誌情報 |
THERIOGENOLOGY 巻 62, 号 5, p. 897-905, 発行日 2004-09-01 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0093-691X | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1016/j.theriogenology.2003.12.008 | |||||||||||
| リンクURL | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | http://www.sciencedirect.com/science/journal/0093691X | http://www.sciencedirect.com/science/journal/0093691X | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Potential methods for cryopreservation of mouse spermatozoa are freeze-drying, desiccation, and suspension in EGTA Tris–HCl buffered solution (ETBS: 50 mM NaCl, 50 mM EGTA, and 10 mM Tris–HCl). To determine the duration that mouse spermatozoa suspended in ETBS-based solutions could retain their normal characteristics without freezing, spermatozoa collected from the cauda epididymis were suspended in ETBS or in ETBS supplemented with the antioxidants, dimethyl sulfoxide (DMSO), or _DL-α-tocopherol acetate (Vitamin E acetate; VEA) diluted in DMSO, then held at ambient temperature (22–24 °C) for up to 9 days. When oocytes were injected with spermatozoa preserved in ETBS alone, activation rates of oocytes and chromosome integrity at the first cleavage metaphase decreased at 1 day (P<0.001) and 2–4 days (P<0.01) following treatment. When oocytes were injected with spermatozoa preserved in ETBS supplemented with DMSO or VEA/DMSO, chromosome integrity did not decrease significantly (through 9 days of preservation). Although DMSO maintained sperm chromosome integrity more effectively than VEA/DMSO up to 2–4 days (91 and 67%, normal karyotypes in DMSO and VEA/DMSO, respectively), VEA/DMSO helped to maintain the ability of spermatozoa to activate oocytes, but did not enhance the maintenance of sperm chromosome integrity. These results suggested that deterioration of spermatozoa preserved in ETBS alone was delayed by supplementation with antioxidants. | |||||||||||
| 注記 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 注記 | author | |||||||||||
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| 内容記述タイプ | Other | |||||||||||
| 資源タイプ | text | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| ID(XooNIps) | ||||||||||||
| 15251241 | ||||||||||||
| 閲覧数(XooNIps) | ||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||
| 1032 | ||||||||||||
