| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2009-03-11 |
| タイトル |
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タイトル |
Lipid peroxidation and 4-hydroxy-2-nonenal formation by copper ion bound to amyloid-β peptide |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
中村, 正雄
Hayashi, T
Shishido, N
Nakayama, K
Nunomura, A
Smith, MA
Perry, G
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| 著者 ローマ字 |
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Nakamura, Masao |
| 書誌情報 |
Free Radical Biology and Medicine
巻 43,
号 11,
p. 1552-1559,
発行日 2007-12-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0891-5849 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.freeradbiomed.2007.08.013 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://dx.doi.org/10.1016/j.freeradbiomed.2007.08.013 | http://dx.doi.org/10.1016/j.freeradbiomed.2007.08.013 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The lipid peroxidation product 4-hydroxy-2-nonenal (HNE) is proposed to be a toxic factor in the pathogenesis of Alzheimer’s disease. The primary products of lipid peroxidation are phospholipid hydroperoxides and degraded reactive aldehydes, such as HNE, as secondary peroxidation products. In this study, we investigated the role of amyloid-β peptide (Aβ) in the formation of phospholipid hydroperoxides and HNE by copper ion bound to Aβ. The Aβ_<1-42>-Cu^<2+> (1:1 molar ratio) complex showed an activity to form phospholipid hydroperoxides from phospholipid, 1-palmitoyl-2-linoleoyl phosphatidylcholine (PLPC), through Cu^<2+> reduction in the presence of ascorbic acid. The phospholipid hydroperoxides were considered to be racemic mixture of 9-hydroperoxide and 13-hydroperoxide of linoleoyl residue. When Cu^<2+> was bound to two molar equivalents of Aβ_<1-42> (2 Aβ_<1-42>-Cu^<2+>), lipid peroxidation was inhibited. HNE was generated from one of the phospholipid hydroperoxides, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl) phosphatidylcholine (PLPC-OOH), by free Cu^<2+> in the presence of ascorbic acid through Cu^<2+> reduction and degradation of PLPC-OOH. HNE generation was markedly inhibited by equimolar concentrations of Aβ_<1-40> (92%) and Aβ_<1-42> (92%). However, Aβ_<1-42> binding two or three molar equivalents of Cu^<2+> (Aβ_<1-42>-2Cu^<2+>, Aβ_<1-42>-3Cu^<2+>) acted as a pro-oxidant to form HNE from PLPC-OOH. These findings suggest that, at moderate concentrations of copper, Aβ acts primarily as an antioxidant to prevent Cu^<2+>-catalyzed oxidation of biomolecules, but that, in the presence of excess copper, pro-oxidant complexes of Aβ with Cu^<2+> are formed. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
author |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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17964426 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1271 |
1611.pdf (386.5 kB)
