{"created":"2023-06-19T11:21:11.467688+00:00","id":1341,"links":{},"metadata":{"_buckets":{"deposit":"6f02045c-2f9c-4dfd-9562-38abf612027a"},"_deposit":{"created_by":3,"id":"1341","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1341"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00001341","sets":["6:29:30","7","7:24"]},"author_link":["4325","4326","4327","4328","4329"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"43","bibliographicPageEnd":"29155","bibliographicPageStart":"29144","bibliographicVolumeNumber":"283","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.ncbi.nlm.nih.gov/pubmed/18728008 | http://www.ncbi.nlm.nih.gov/pubmed/18728008","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tyr(122)-hydrophobic cluster (Y122-HC) is an interaction network formed by the top part of the second transmembrane helix and the cytoplasmic actuator and phosphorylation domains of sarcoplasmic reticulum Ca(2+)-ATPase. We have previously found that Y122-HC plays critical roles in the processing of ADP-insensitive phosphoenzyme (E2P) after its formation by the isomerization from ADP-sensitive phosphoenzyme (E1PCa(2)) (Wang, G., Yamasaki, K., Daiho, T., and Suzuki, H. (2005) J. Biol. Chem. 280, 26508-26516). Here, we further explored kinetic properties of the alanine-substitution mutants of Y122-HC to examine roles of Y122-HC for Ca(2+) release process in E2P. In the steady state, the amount of E2P decreased so that of E1PCa(2) increased with increasing lumenal Ca(2+) concentration in the mutants with K(0.5) 110-320 microm at pH 7.3. These lumenal Ca(2+) affinities in E2P agreed with those estimated from the forward and lumenal Ca(2+)-induced reverse kinetics of the E1PCa(2)-E2P isomerization. K(0.5) of the wild type in the kinetics was estimated to be 1.5 mM. Thus, E2P of the mutants possesses significantly higher affinities for lumenal Ca(2+) than that of the wild type. The kinetics further indicated that the rates of lumenal Ca(2+) access and binding to the transport sites of E2P were substantially slowed by the mutations. Therefore, the proper formation of Y122-HC and resulting compactly organized structure are critical for both decreasing Ca(2+) affinity and opening the lumenal gate, thus for Ca(2+) release from E2PCa(2). Interestingly, when K(+) was omitted from the medium of the wild type, the properties of the wild type became similar to those of Y122-HC mutants. K(+) binding likely functions via producing the compactly organized structure, in this sense, similarly to Y122-HC.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"author","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M804596200","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"18728008"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2282"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1255"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Yamasaki, Kazuo"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 和生"},{"creatorName":"ヤマサキ, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4325","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, G"}],"nameIdentifiers":[{"nameIdentifier":"4326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daiho, T"}],"nameIdentifiers":[{"nameIdentifier":"4327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Danko, S"}],"nameIdentifiers":[{"nameIdentifier":"4328","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, H"}],"nameIdentifiers":[{"nameIdentifier":"4329","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"1605.pdf","filesize":[{"value":"3.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1605.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/1341/files/1605.pdf"},"version_id":"3e4e887e-97aa-4534-93a8-97cf15dd9f8b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Roles of Tyr122-hydrophobic cluster and K+ binding in Ca2+ -releasing process of ADP-insensitive phosphoenzyme of sarcoplasmic reticulum Ca2+ -ATPase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Roles of Tyr122-hydrophobic cluster and K+ binding in Ca2+ -releasing process of ADP-insensitive phosphoenzyme of sarcoplasmic reticulum Ca2+ -ATPase","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["7","24","30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-11"},"publish_date":"2009-03-11","publish_status":"0","recid":"1341","relation_version_is_last":true,"title":["Roles of Tyr122-hydrophobic cluster and K+ binding in Ca2+ -releasing process of ADP-insensitive phosphoenzyme of sarcoplasmic reticulum Ca2+ -ATPase"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:24:13.607649+00:00"}