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Transforming Growth Factor-β Signaling Cascade Induced by Mechanical Stimulation of Fluid Shear Stress in Cultured Corneal Epithelial Cells. 宇都宮, 嗣了 ウツノミヤ, ツギアキ open access PMID：27898984 Purpose: \nBecause blinking is regarded as mechanical stimulation of fluid shear stress on the corneal epithelial cells, we investigated the effects of fluid shear stress on cultured human corneal epithelial cells (HCECs). Methods: \nThe HCECs were exposed to shear stress (0, 1.2, 12 dyne/cm2) with the parallel-plate type of flow chamber. Wound healing, cellular proliferation, growth factor expression, TGF-β1 concentration in the culture supernatant, and phosphorylation of SMAD2 were investigated. Results: \nMonolayers of HCECs exposed to shear stress had delayed wound healing and decreased proliferation compared with those of the static control (0 dyne/cm2). With increasing shear stress, TGF-β1 expression and phosphorylation of SMAD2 increased significantly, but the levels of total TGF-β1 in the culture supernatant decreased significantly. Delayed wound healing, decreased proliferation, and phosphorylation of the SMAD2 by shear stress were canceled out with a TGF-β receptor inhibitor. Conclusions: \nFluid shear stress on the HCECs affected TGF-β signaling, which was associated with delayed wound healing. Mechanical stress by blinking might involve TGF-β signaling, and activation of TGF-β might be a key factor in wound healing of the corneal epithelium. Further studies should investigate the molecular mechanism of shear stress-induced activation of TGF-β. application/pdf application/pdf 2016-11-01 eng doctoral thesis VoR https://asahikawa-med.repo.nii.ac.jp/records/5657 10.1167/iovs.16-20638. 0146-0404 Investigative ophthalmology and visual science 57 14 6382 6388 甲509 博士（医学） 2017-03-24 旭川医科大学 https://asahikawa-med.repo.nii.ac.jp/record/5657/files/6707.pdf application/pdf 909.3 kB 2021-07-06