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Observation of Zn-photoprotoporphyrin red Autofluorescence in human bronchial cancer using color-fluorescence endoscopy 大崎, 能伸 オオサキ, ヨシノブ 佐々木, 高明 ササキ, タカアキ 遠藤, 哲史 エンドウ, サトシ 北田, 正博 キタダ, マサヒロ 奥村, 俊介 オクムラ, シュンスケ 平井, 理子 ヒライ, ノリコ 風林, 佳大 カゼバヤシ, ヨシヒロ 豊嶋, 恵理 トヨシマ, エリ 山本, 泰司 ヤマモト, ヤスシ 竹山, 周良 タケヤマ, カネヨシ 中島, 進 ナカジマ, ススム 阪田, 功 サカタ, イサオ Autofluorescence Endoscopy Photodynamic diagnosis Prophyrin Zn-photoprotoporphyrin PMID：28441937 BACKGROUND: \nWe observed red autofluorescence emanating from bronchial cancer lesions using a sensitive color-fluorescence endoscopy system. We investigated to clarify the origin of the red autofluorescence. METHODS: \nThe wavelengths of the red autofluorescence emanating from lesions were measured in eight patients using a spectrum analyzer and compared based on pathologic findings. Red autofluorescence at 617.3, 617.4, 619.0, and 617.1 nm was emitted by normal bronchus, inflamed tissue, tissue exhibiting mild dysplasia, and malignant lesions, respectively. Protoporphyrin, uroporphyrin, and coproporphyrin, the major porphyrin derivatives in human blood, were purchased to determine which porphyrin derivative is the source of red fluorescence when acquired de novo. We synthesized photoporphyrin, Zn-protoporphyrin and Zn-photoprotoporphyrin from protoporphyrin. RESULTS: \nCoproporphyrin and uroporphyrin emitted only weak fluorescence. Fluorescence was emitted by our synthesized Zn-photoprotoporphyrin at 625.5 nm and by photoprotoporphyrin at 664.0 nm. CONCLUSIONS: \nFrom these results, we conclude that Zn-photoprotoporphyrin was the source of the red autofluorescence observed in bronchial lesions. Zn-protoporphyrin is converted to Zn-photoprotoporphyrin by radiation with excitation light. Our results suggest that red autofluorescence emanating from Zn-photoprotoporphyrin in human tissues could interfere with photodynamic diagnosis using porphyrin derivatives such as Photofrin® and Lazerphyrin® with a sensitive endoscopy system, because color cameras cannot differentiate Zn-photoprotoporphyrin red fluorescence from that of other porphyrin derivatives. text application/pdf 2017-04-01 eng journal article VoR https://asahikawa-med.repo.nii.ac.jp/records/5895 10.1186/s12885-017-3277-6 1471-2407 BMC Cancer 17 1 289 https://asahikawa-med.repo.nii.ac.jp/record/5895/files/7004.pdf application/pdf 668.1 kB 2021-07-06